Abstract: The principles, application and industrialization of biological desulphurisation methods are reviewed including biological filtering, biological trickling and biological scrubbing. At the same time, related researches on the biotreatment of H2S containing gas by using these biological desulphurisation methods are described. These biotreatment methods have been widely used in industry out of China for the purification of H2S containing gases such as natural gas, synthetic gas and fuel gas as well as and from ethylamine is made to absorb renewable process acid gas desulphurization. Mortherland relatively mature，industrial application instance has many been realized preliminary，such as emission desulphurisation，wet desulphurisation loop fluid circulation and regeneationzaisheng，etc. With Compared with mature development of the physical and chemical methods, the biological compared to the biochip method has its special superiority，such as low - cost，second pollution small，and so on.