利用树干毕赤酵母发酵玉米秸秆制备燃料酒精

CIESC Journal

利用树干毕赤酵母发酵玉米秸秆制备燃料酒精

张强1，戈兴炜2，Anders Thygesen3，Anne Belinda Thomsen3

1长春理工大学生命科学技术学院，吉林长春 130022；2长春理工大学校医院，吉林长春 130022；3Biosystems Division，Ris? DTU，Technical University of Denmark，Denmark

出版日期:2010-12-24 发布日期:2010-12-22

Fuel ethanol production from corn stover by Pichia stipitis

ZHANG Qiang1，GE Xingwei2，Anders Thygesen3，Anne Belinda Thomsen3

1School of Life Science and Technology，Changchun University of Science and Technology，Changchun 130022，Jilin，China；2Hospital Affiliated to Changchun University of Science and Technology，Changchun 130022，Jilin，China；3 Biosystems Division，Ris? DTU，Technical University of Denmark，Denmark

Online:2010-12-24 Published:2010-12-22

摘要/Abstract

摘要：

采用湿热预处理（195 ℃，15 min）与同步糖化发酵对玉米秸秆制备酒精进行了研究。结果表明：玉米秸秆经过湿热预处理后，86.5%纤维素保留在滤饼中，而大部分半纤维素被溶解。在底物质量体积含量50 g/L，温度30 ℃，pH值5.5，摇床转速130 r/min条件下，树干毕赤酵母（Pichia stipitis）利用预处理后的玉米秸秆经过192 h同步糖化发酵，酒精浓度达到了12.12 g/L，对应的酒精产量和生产效率分别为0.34 g/g（葡萄糖+木糖）和0.065 g/(L·h)。该项研究为工业化生产打下了基础。

Abstract:

Hydrothermal pretreatment（195 ℃，15 min）and simultaneous saccharification and fermentation（SSF）were adopted to produce ethanol from corn stover. Results showed that 86.5% of cellulose was remained in the solid cake and most of hemicellulose was solubilized after the hydrothermal pretreatment. The highest ethanol concentration of 12.12 g/ L was achieved at initial pH of 5.5，temperature of 30 ℃ and 130 r/min shaking rate with substrate concentration of 50 g/L by Pichia stipitis after 192 h. The corresponding alcohol yield and volumetric productivity were 0.34 g/g（glucose + xylose）and 0.065 g/(L·h) respectively.

张强1，戈兴炜2，Anders Thygesen3，Anne Belinda Thomsen3. 利用树干毕赤酵母发酵玉米秸秆制备燃料酒精 [J]. CIESC Journal.

ZHANG Qiang1，GE Xingwei2，Anders Thygesen3，Anne Belinda Thomsen3. Fuel ethanol production from corn stover by Pichia stipitis[J]. .