Chinese Journal of Chemical Engineering ›› 2012, Vol. 20 ›› Issue (5): 930-936.

• 生物技术与生物工程 • 上一篇    下一篇

长双歧杆菌BIOMA 5920的冻干保护剂配方的优化

杨婵媛1,2; 朱晓丽1,3; 范代娣1,2; 米钰1,2; 骆艳娥1,2; 惠俊峰1,2; 苏然1,2   

  1. 1 Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest Uni-versity, Xi’an 710069, China 2 Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi’an 710069, China 3 Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Urban and Environmental Science, Northwest University, Xi’an 710069, China
  • 收稿日期:2011-03-06 修回日期:2011-09-29 出版日期:2012-10-28 发布日期:2011-09-29

Optimizing the chemical compositions of protective agents for freeze-drying Bifidobacterium longum BIOMA 5920

YANG Chanyuan1,2; ZHU Xiaoli1,3; FAN Daidi1,2; MI Yu1,2; LUO Yan’e1,2; HUI Junfeng1,2; SU Ran1,2   

  1. 1 Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest Uni-versity, Xi’an 710069, China 2 Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi’an 710069, China 3 Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Urban and Environmental Science, Northwest University, Xi’an 710069, China
  • Received:2011-03-06 Revised:2011-09-29 Online:2012-10-28 Published:2011-09-29

摘要： Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of Bifidobacterium longum BIOMA 5920 during freeze-drying. Through the comparative analysis of single protectant, the complex protective agents show better effect on the Bifidobacterium viability. Human-like collagen (HLC), trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows: HLC 1.23%, trehalose 11.50% and glycerol 4.65%. Under this formula, the viability is 88.23%, 39.67% higher in comparison to the control. The viable count is 1.07×109 cfu•g?1, greatly exceeding the minimum viable count requirement (106 cfu•g?1).

关键词: Bifidobacterium, freeze-drying, viability, protective agent, response surface methodology

Abstract: Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of Bifidobacterium longum BIOMA 5920 during freeze-drying. Through the comparative analysis of single protectant, the complex protective agents show better effect on the Bifidobacterium viability. Human-like collagen (HLC), trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows: HLC 1.23%, trehalose 11.50% and glycerol 4.65%. Under this formula, the viability is 88.23%, 39.67% higher in comparison to the control. The viable count is 1.07×109 cfu•g?1, greatly exceeding the minimum viable count requirement (106 cfu•g?1).

Key words: Bifidobacterium, freeze-drying, viability, protective agent, response surface methodology

版权所有 © 2019 《化工学报》编辑部
北京市东城区青年湖南街13号（100011）
电话：010-64519489，010-64519362，010-64519485，010-64519490，010-64519451
E-Mail：hgxb@cip.com.cn
京ICP备12046843号-7
京公网安备 11010102001995号
本系统由北京玛格泰克科技发展有限公司设计开发