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黏红酵母CCZU-G5选择性加氢柠檬醛合成香叶醇

朱 劼,汪 云,李晨旸,符梦婷,王利群,何玉财   

  1. 常州大学制药与生命科学学院,江苏 常州 213164
  • 出版日期:2013-05-05 发布日期:2013-05-25

Selective hydrogenation of citral to geraniol using Rhodotorula mucilaginosa CCZU-G5

ZHU Jie,WANG Yun,LI Chenyang,FU Mengting,WANG Liqun,HE Yucai   

  1. School of Pharmaceutical Engineering and Life Science,Changzhou University,Changzhou 213164,Jiangsu,China
  • Online:2013-05-05 Published:2013-05-25

摘要: 以黏红酵母CCZU-G5为菌株,通过对菌体培养时间、辅底物葡萄糖浓度、底物柠檬醛初始用量、菌体浓度及反应时间等条件的研究,考察其对柠檬醛选择性加氢反应的影响。结果表明,当培养28 h后,菌体达到对数生长期末期,此时菌体数量及活力处于较高水平。利用此时的菌体进行加氢反应过程中,最佳反应条件为:辅底物葡萄糖浓度40 g/L,底物柠檬醛添加浓度4 g/L,菌体浓度0.4 g/mL,反应时间22 h。在此条件下,柠檬醛转化率及对香叶醇的选择性分别达88.6%与95.2%。此研究为生物合成香叶醇的工业化奠定基础。

关键词: 黏红酵母, 柠檬醛选择性加氢, 香叶醇

Abstract: Biological conversion of citral to geraniol was studied using Rhodotorula mucilaginosa CCZU-G5 as fermentation strain. The effects of culture time,co-substrate concentration,initial substrate concentration,cell quantity and the reaction time on selective hydrogenation of citral to geraniol were investigated to determine the optimal fermentation conditions. The results showed that after 28 h culturing,yeast cells reached the end of the logarithmic growth phase,in which the quantity and activity of the cells were at a high level. In the process of biological conversion,the optimal fermentation conditions were as follows:glucose concentration 40 g/L,initial citral concentration 4 g/L,yeast cell concentration 0.4 g/mL and reaction time 22 h. Under these conditions,citral conversion and geraniol selectivity reached 88.6% and 95.2%,respectively. The study lays the foundation for commercialized biosynthesis of geraniol.

Key words: Rhodotorula mucilaginosa, selective hydrogenation of citral, geraniol