不同营养下混合菌种微生物污垢生长机理与交互作用

doi:10.3969/j.issn.0438-1157.2013.03.035

化工学报 ›› 2013, Vol. 64 ›› Issue (3): 1036-1041.DOI: 10.3969/j.issn.0438-1157.2013.03.035

不同营养下混合菌种微生物污垢生长机理与交互作用

杨倩鹏¹, 陈晓东², 田磊¹, 史琳¹

1. 清华大学热能工程系,热科学与动力工程教育部重点实验室,北京 100084;
2. 厦门大学化学化工学院化学工程与生物工程系,福建厦门 361005

收稿日期:2012-05-23 修回日期:2012-06-27 出版日期:2013-03-05 发布日期:2013-03-05
通讯作者: 史琳
作者简介:杨倩鹏(1989—),男,博士研究生。
基金资助:
国家自然科学基金项目(50976060);国家重点基础研究发展计划项目(2010CB227305)。

Growth and interaction mechanism of multi-strain biofouling under different nutrient levels

YANG Qianpeng¹, CHEN Xiaodong², TIAN Lei¹, SHI Lin¹

1. Key Laboratory for Thermal Science and Power Engineering of Ministry of Education, Department of Thermal Engineering, Tsinghua University, Beijing 100084, China;
2. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian, China

Received:2012-05-23 Revised:2012-06-27 Online:2013-03-05 Published:2013-03-05
Supported by:
supported by the National Natural Science Foundation of China(50976060)and the National Basic Research Program of China(2010CB227305).

摘要/Abstract

摘要： 再生水源热泵能有效回用废热,但再生水换热器的微生物污垢问题一直没有完全解决,严重影响换热效率和系统安全。利用长期监测并且进行16S rRNA鉴定得到的再生水及其污垢中两种主要代表菌属芽孢杆菌属和气单胞菌属,在实验室内配制模拟流体进行混合菌种微生物污垢实验。通过在特制的机理流道上进行不同菌种比例和不同营养物质浓度的污垢生长实验,得出芽孢杆菌属和气单胞菌属的单独生长规律和混合生长规律,总结了混合生长时两种菌属的协同和抑制规律,同时考察了营养物质浓度对生长规律和交互作用的影响。结果表明:芽孢杆菌属成垢能力较强,细胞分裂速度高,耐贫营养能力强;气单胞菌属成垢能力弱于芽孢杆菌属,但分泌物生成速率高,可以与芽孢杆菌属协同生长;两种菌属交互作用在不同阶段和条件下,协同与抑制作用共存并轮流占据主导。

关键词: 微生物污垢, 混合菌种, 生长机理, 协同与抑制

Abstract: Heat pump system is an effective method for heat recovery of a treated sewage source,but biofouling on heat exchanger is still a problem to be solved,and may affect seriously efficiency and security of the system.By long term monitoring and 16S rRNA identification,two major bacteria genus,Bacillus sp. and Aeromonas sp.,were selected for multi-strain biofouling experiment of a model fluid in laboratory.By the experiment of foul growth performed on a purpose made channel at various bacteria ratios and nutrient levels,the regular patterns of single and mixed microbe growth of Bacillus sp. and Aeromonas sp. were revealed,their synergy and inhibition interaction summarized,and the effect of nutrient levels on their growth mechanism and interaction also investigated.The results indicated that the Bacillus sp. has strong ability in biofouling,in high multiple rates,and high tolerance to poor nutrient level.The biofouling of Aeromonas sp. is weak relatively,but high secretion rate that may help for the synergy growth with Bacillus sp.Their synergy and inhibition interactions co-exist and dominate in turn under different growth stage and condition.

Key words: biofouling, multi-strain, growth mechanism, synergy and inhibition interactions

中图分类号:

TK01

杨倩鹏, 陈晓东, 田磊, 史琳. 不同营养下混合菌种微生物污垢生长机理与交互作用[J]. 化工学报, 2013, 64(3): 1036-1041.

YANG Qianpeng, CHEN Xiaodong, TIAN Lei, SHI Lin. Growth and interaction mechanism of multi-strain biofouling under different nutrient levels[J]. CIESC Journal, 2013, 64(3): 1036-1041.

参考文献 15

[1]	Tian Lei(田磊),Yang Qianpeng(杨倩鹏),Shi Lin(史琳),Chen Jinchun(陈金春).Evolution of biofouling on a vertical stainless steel surface caused by Bacillus subtilis under heat pump conditions[J].CIESC Journal(化工学报),2012,63(1):230-236
[2]	Tian Lei,Chen Xiaodong,Yang Qianpeng,Chen Jinchun,Shi Lin,Li Qiong.Effect of calcium ions on the evolution of biofouling by Bacillus subtilis in plate heat exchangers simulating the heat pump system used with treated sewage in the 2008 Olympic Village[J].Colloids and Surfaces B:Biointerfaces,2012,94:309-316
[3]	Tian Lei,Chen Xiaodong,Yang Qianpeng,Chen Jinchun,Li Qiong,Shi Lin.Effect of silica dioxide particles on the evolution of biofouling by Bacillus subtilis in plate heat exchangers relevant to a heat pump system used with treated sewage[J].Chemical Engineering Journal,2012,188:47-56
[4]	Xu Zhiming(徐志明),Guo Jinsheng(郭进生),Huang Xing(黄兴),Zhang Zhongbin(张仲彬).Relationship between water quality parameters and fouling in plate heat exchangers[J].CIESC Journal(化工学报),2011,62(2):344-347
[5]	Xu Zhiming(徐志明),Wen Xiaoqiang(文孝强),Zheng Jiaoli(郑娇丽),Guo Jinsheng(郭进生),Huang Xing(黄兴).Cooling water fouling resistance prediction of plate heat exchanger based on partial least squares regression[J].CIESC Journal(化工学报),2011,62(6):1531-1536
[6]	Yu Dayu(于大禹),Yin Xu(尹旭),Zhang Jing(张静),Gao Bo(高波),Wang Gang(王刚),Cao Shengxian(曹生现),Yang Shanrang(杨善让),Xu Zhiming(徐志明).Factors affecting microbial fouling formation in simulated recirculating cooling-water system[J].CIESC Journal(化工学报),2011,62(12):3503-3510
[7]	Olivier Habimana,Laurent Guillier,Saulius Kulakauskas,Romain Briandet.Spatial competition with Lactococcus lactis in mixed-species continuous-flow biofilms inhibits Listeria monocytogenes growth[J].Biofouling,2011,27(9):1065-1072
[8]	Greetje A A Castelijn,Stijn van der Veen,Marcel H Zwietering,Roy Moezelaar,Tjakko Abee.Diversity in biofilm formation and production of curli fimbriae and cellulose of Salmonella typhimurium strains of different origin in high and low nutrient medium[J].Biofouling,2012,28(1):51-63
[9]	Hiren Joshi M,Balamurugan P,Venugopalan V P,Rao T S.Dense fouling in acid transfer pipelines by an acidophilic rubber degrading fungus[J].Biofouling,2011,27(6):621-629
[10]	Monteiro D R,Gorup L F,Silva S,Negri M,de Camargo E R,Oliveira R,Barbosa D B,Henriques M.Silver colloidal nanoparticles:antifungal effect against adhered cells and biofilms of Candida albicans and Candida glabrata[J].Biofouling,2011,27(7):711-719
[11]	Federico Rossi,Ernesto Micheletti,Laura Bruno,Siba P Adhikary,Patrizia Albertano,Roberto De Philippis. Characteristics and role of the exocellular polysaccharides produced by five cyanobacteria isolated from phototrophic biofilms growing on stone monuments[J].Biofouling,2012,28(2):215-224
[12]	Liesse Iyamba J M,Seil M,Devleeschouwer M,Takaisi Kikuni N B,Dehaye J P.Study of the formation of a biofilm by clinical strains of Staphylococcus aureus[J].Biofouling,2011,27(8):811-821
[13]	Sarkisova S,Patrauchan M A,Berglund D,Nivens D E,Franklin M J.Calcium-induced virulence factors associated with the extracellular matrix of Mucoid Pseudomonas aeruginosa biofilm[J].American Society for Microbiology,2005,187(13):4327-4337
[14]	Soojin Shim,Seok Hoon Hong,Yongsug Tak,Jeyong Yoon.Prevention of Pseudomonas aeruginosa adhesion by electric currents[J].Biofouling,2011,27(2):217-224
[15]	Thomas K Wood,Andrés F,González Barrios,Moshe Herzberg,Jintae Lee.Motility influences biofilm architecture in Escherichia coli[J].Appl. Microbiol. Biotechnol.,2006,72:361-367

不同营养下混合菌种微生物污垢生长机理与交互作用

Growth and interaction mechanism of multi-strain biofouling under different nutrient levels

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献 15

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘坐东, 李斯琪, 邢维维, 徐志明. 板式换热器Ni-P-TiO₂复合纳米镀层微生物污垢特性[J]. 化工学报, 2020, 71(8): 3535-3544.
[2]	徐志明, 胡春阳, 王景涛, 刘坐东. 板式换热器黏液形成菌与CaCO₃混合污垢实验[J]. 化工学报, 2017, 68(1): 72-78.
[3]	徐志明, 沈艺雯, 张一龙, 刘坐东, 王景涛, 王宇航. 基于Kern-Seaton模型结合Capdeville生物膜增长体系建立的微生物污垢模型[J]. 化工学报, 2016, 67(7): 2998-3003.
[4]	王海洋, 杜艳妮, 李振方, 宋晓鹏, 谭端明, 龚俊波. 硫酸氢氯吡格雷球形结晶工艺及其生长机理[J]. 化工学报, 2015, 66(9): 3633-3639.
[5]	李国华, 孙海标, 喻洋, 谢伟淼. 火柴棒状纳米碳管的制备及其生长机理[J]. 化工学报, 2015, 66(9): 3801-3807.
[6]	崔贤羡, 管卉, 黄民. 高结晶度ZSM-5分子筛合成及分子动力学模拟[J]. 化工学报, 2013, 64(4): 1493-1498.
[7]	关晓辉，崔长龙，曹生现，鲁敏，付俊杰，孙玲玲，杨善让. 不锈钢缩放管中典型致垢微生物致垢能力[J]. 化工进展, 2013, 32(06): 1429-1434.
[8]	田磊, 杨倩鹏, 史琳, 陈金春. 热泵工况下竖直不锈钢表面微生物污垢动态生长行为[J]. 化工学报, 2012, 63(1): 230-236.
[9]	于大禹1，尹旭1，张静1，高波1，王刚1，曹生现2，杨善让2，徐志明SPAN. 基于模拟循环冷却装置的微生物污垢形成的影响因素[J]. CIESC Journal, 2011, 62(12): 3503-3510.
[10]	于大禹1，2，张静1，2，尹旭1，2，王刚1，2，张艳辉1，曹生现1. 循环冷却水管路微生物污垢形成的动态模拟 [J]. CIESC Journal, 2010, 29(11): 2193-.
[11]	王晓东, 张宝泉, 刘秀凤. 取向TS-1分子筛膜的合成与结构引导机理 [J]. 化工学报, 2007, 58(9): 2408-2411.
[12]	郎林;张宝泉;刘秀凤 . 在玻璃上合成有取向的silicalite-1分子筛膜及其生长机理 [J]. CIESC Journal, 2006, 57(9): 2229-2232.
[13]	刘秀凤;郎林;张宝泉;Y.S.LIN. 在壳聚糖改性的硅锆复合中间层上原位水热合成b-轴取向silicalite-1分子筛膜 [J]. CIESC Journal, 2006, 57(4): 1019-1022.
[14]	蒋海洋, 张宝泉, 林跃生, 李永丹. 无模板剂二次生长法合成MFI分子筛膜的生长机理 [J]. 化工学报, 2005, 56(6): 1141-1143.
[15]	赖琛, 唐绍裘, 王迎军, 魏小焜, 张世英. 微乳法合成磷酸钙纳米纤维及其机理探讨 [J]. 化工学报, 2005, 56(5): 942-946.