Effect of Pore-size of Mesoporous SBA-15 on Adsorption of Bovine Serum Albumin and Lysozyme Protein

CIESC Journal ›› 2010, Vol. 18 ›› Issue (3): 493-499.

Effect of Pore-size of Mesoporous SBA-15 on Adsorption of Bovine Serum Albumin and Lysozyme Protein

刁香^1,2, 王玉军¹, 赵俊琦¹, 朱慎林¹

1. The State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;
2. Department of Chemical Engineering, Zibo Vocational Institute, Zibo 255314, China

收稿日期:2009-09-01 修回日期:2010-03-30 出版日期:2010-06-28 发布日期:2010-06-28
通讯作者: WANG Yujun,E-mail:wangyujun@mail.tsinghua.edu.cn
基金资助:
Supported by the National Basic Research Program of China(2007CB714302);the Outstanding Young Teacher Visiting Scholar Program of Shandong Province

Effect of Pore-size of Mesoporous SBA-15 on Adsorption of Bovine Serum Albumin and Lysozyme Protein

DIAO Xiang^1,2, WANG Yujun¹, ZHAO Junqi¹, ZHU Shenlin¹

1. The State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;
2. Department of Chemical Engineering, Zibo Vocational Institute, Zibo 255314, China

Received:2009-09-01 Revised:2010-03-30 Online:2010-06-28 Published:2010-06-28
Supported by:
Supported by the National Basic Research Program of China(2007CB714302);the Outstanding Young Teacher Visiting Scholar Program of Shandong Province

摘要/Abstract

摘要： In this paper,the effects of pore-size of SBA-15 on the adsorption kinetics and equilibrium of large protein molecules Bovine serum albumin(BSA)and lysozyme(LYS)have been investigated.The mesoporous molecular sieve SBA-15 with six different pore sizes were synthesized with P123 triblock copolymer as the template agent,and 1,3,5-trimethylbenzene(TMB)and isopropyl alcohol as the pore-expanding agent.The samples were characterized by N₂ adsorption/desorption,Scanning Electron Microscopy(SEM),Transmission Electron Microscopy(TEM)and X-Ray Diffraction(XRD).It is found that BSA and LYS were adsorbed rapidly on SBA-15 materials with large pores.The BSA adsorption capacity of sieve with the pore diameter of 21.4 nm reached 500 mg·g^-1 within 25 minutes.However,if the pore diameter was smaller than 14 nm,the BSA adsorption capacity of the sieve was only about 220 mg·g^-1.The adsorption equilibrium data fits in the Langmuir model,where the coefficient of effective use of specific area of mesoporous molecular sieve was found to be 0.03,0.18,0.37 and 0.48,corresponding to the pore diameter of 10.1 nm,13.2 nm,15.4 nm and 21.4 nm,respectively.The equilibrium loading amount of LYS on SBA-15 materials with pore size of 15.4 nm could be up to 1000 mg·g^-1.The coefficient of effective use of surface area of mesoporous molecular sieve with diameter of 3.9 nm,7.4 nm,10.1 nm,13.2 nm and 15.4 nm was 0.10,0.47,0.56,0.71 and 0.79,respectively.It is also noted that greater pore size of mesoporous molecular sieve would lead to a higher coefficient of effective use of surface area.

关键词: pore-size, mesoporous, SBA-15, protein adsorption

Abstract: In this paper,the effects of pore-size of SBA-15 on the adsorption kinetics and equilibrium of large protein molecules Bovine serum albumin(BSA)and lysozyme(LYS)have been investigated.The mesoporous molecular sieve SBA-15 with six different pore sizes were synthesized with P123 triblock copolymer as the template agent,and 1,3,5-trimethylbenzene(TMB)and isopropyl alcohol as the pore-expanding agent.The samples were characterized by N₂ adsorption/desorption,Scanning Electron Microscopy(SEM),Transmission Electron Microscopy(TEM)and X-Ray Diffraction(XRD).It is found that BSA and LYS were adsorbed rapidly on SBA-15 materials with large pores.The BSA adsorption capacity of sieve with the pore diameter of 21.4 nm reached 500 mg·g^-1 within 25 minutes.However,if the pore diameter was smaller than 14 nm,the BSA adsorption capacity of the sieve was only about 220 mg·g^-1.The adsorption equilibrium data fits in the Langmuir model,where the coefficient of effective use of specific area of mesoporous molecular sieve was found to be 0.03,0.18,0.37 and 0.48,corresponding to the pore diameter of 10.1 nm,13.2 nm,15.4 nm and 21.4 nm,respectively.The equilibrium loading amount of LYS on SBA-15 materials with pore size of 15.4 nm could be up to 1000 mg·g^-1.The coefficient of effective use of surface area of mesoporous molecular sieve with diameter of 3.9 nm,7.4 nm,10.1 nm,13.2 nm and 15.4 nm was 0.10,0.47,0.56,0.71 and 0.79,respectively.It is also noted that greater pore size of mesoporous molecular sieve would lead to a higher coefficient of effective use of surface area.

Key words: pore-size, mesoporous, SBA-15, protein adsorption

刁香, 王玉军, 赵俊琦, 朱慎林. Effect of Pore-size of Mesoporous SBA-15 on Adsorption of Bovine Serum Albumin and Lysozyme Protein[J]. CIESC Journal, 2010, 18(3): 493-499.

DIAO Xiang, WANG Yujun, ZHAO Junqi, ZHU Shenlin. Effect of Pore-size of Mesoporous SBA-15 on Adsorption of Bovine Serum Albumin and Lysozyme Protein[J]. , 2010, 18(3): 493-499.

参考文献

1 Xu, R.R., Pang, W.Q., Yu, J.H., Huo, Q.S., Chen, J.S., Molecular Sieve and Porous Materials Chemistry, Science Press, Beijing, 652(2004).
2 Ajayan,V., Katsuhiko, A., “Preparation of novel mesoporous carbon materials with tunable pore diameters using directly synthesized AlSBA-15 materials”, Chem. lett., 34(5), 674-675(2005).
3 Xu, W., Liao, Y.T., “Formation of CdS nanoparticles within modified MCM-41 and SBA-15”, J. Phys. Chem. B, 106(43), 11127-11131(2002).
4 Hsueh, H.S., Yang, C.T., Zink, J.I., Huang, M.H., “Formation of titanium nitride nanoparticles within mesoporous silica SBA-15”, J. Phys. Chem. B, 109(10), 4404-4409(2005).
5 Yang, C.Z., Huang, M.H., “Formation of arrays of gallium nitride nanorods within mesoporous silica SBA-15”, J. Phys. Chem. B, 109(38), 17842-17847(2005).
6 Zhou, L.H., Hu, J., Xie, S.G., Liu, H.L., “Dispersion of active Au nanoparticles on mesoporous SBA-15 materials”, Chin. J. Chem. Eng., 15(4), 507-511(2007).
7 Wang, A.M., Zhou, C., Wang, H., Shen, S.B., Xue, J.Y., Ouyang, P.K., “Covalent assembly of penicillin acylase in mesoporous silica based on macromolecular crowding theory”, Chin. J. Chem. Eng., 15(6), 788-790(2007).
8 Zhu, Z.Q., Bian, W., “Characterization of tungsten-based catalyst used for selective oxidation of cyclopentene to glutaraldehyde”, Chin. J. Chem. Eng., 16(6), 895-900(2008).
9 Ji, L., Katiyar, A., Pinto, N.G., Jaroniec, M., Smirniotis, P.G., “Al-MCM-41 sorbents for bovine serum albumin:Relation between Al content and performance”, Microporous and Mesoporous Materials, 75(3), 221-229(2004).
10 Zhao, D.Y., Feng, J.L., Huo, Q.S., Melosh, N., Fredrickson, G.H., Chmelka, B.F., Stucky, G.D., “Triblock copolymer syntheses of mesoporous silica with periodic 50 to 300 angstrom pores”, Science, 279(23), 548-552(1998).
11 Zhao, D.Y., Huo, Q.S., Feng, J.L., Chmelka, B.F., Stucky, G.D., “Nonionic triblock and star diblock copolymer and oligomeric surfactant syntheses of highly ordered, hydrothermally stable, mesoporous silica structures'', J. Am. Chem. Soc., 120, 6024-6036(1998).
12 Gao, B., Zhu, G.S., Fu, X.Q., Xin, M.L., Qiu, S.L., “Immobilization and catalytic property of α-chymotrypsin in the channel of silica mesoporous material SBA-15”, Chemical Journal of Chinese Universities, 24(6), 1100-1102(2003).
13 Katiyar, A., Ji, L., Smirniotis, P., Neville, G.P., “Protein adsorption on the mesoporous molecular sieve silicate SBA-15:Effects of pH and pore size”, Journal of Chromatography A, 1069(1), 119-126(2005).
14 Xu, C.J., Qiu, Y., Jia, N.Q., “Immobilization of biomacromolecules based on ordered mesoporous sieve”, Journal of Shanghai Normal University(Natural Sciences), 37(1), 89-92(2008).
15 Lu, Z.X., Ji, S.F., Liu, H., Li, C.Y, “Preparation and characterization of Pt-Sn/SBA-15 catalysts and their catalytic performances for long chain alkane dehydrogenation”, Chin. J. Chem. Eng., 16(5), 740-745(2008).
16 Kresge, C.T., Leonowicz, M.E., Roth, W.J., Vartuli, J.C., B.J.S., “Ordered mesoporous molecular sieves synthesized by a liquid-crystal template mechanism”, Nature, 359, 710-712(1992).
17 Tewodros, A., Bruce, L. R., “Synthesis of nanoparticles via electroless deposition in SBA-15”, Chem. Mater., 17(10), 2481-2483(2005).
18 Shinae, J., Hoon, J.S., Ryong, S.R., “Synthesis of new nanoporous carbon with hexagonally ordered mesostructure”, J. Am. Chem. Soc., 122(43), 10712-10713(2000).
19 Patrick, S.W., Wayne, W.L., Yang, P.D., David, I.M., John, S.L., Jackie, Y.Y., Galen, D., “Microemulsion templating of siliceous mesostructured cellular foams with well-defined ultralarge mesopores”, Chem. Mater., 12(3), 686-696(2000).
20 Zhai, Z., Wang, Y.J., Chen, Y., Luo, G.S., “Fast adsorption and separation of bovine serum albumin and lysozyme using micrometer-sized macromesoporous silica spheres”, J. Sep. Sci., 31(20), 3527-3536(2008).
21 Gregg, S.J., Sing, K.S.W., Adsorption, Surface Area and Porosity, Academic Press, New York(1982).
22 Schmidt-Winkel, P., Lukens, W.W., Zhao, D.Y., Yang, P.D., Chmelka, B.F., Stucky, G.D., “Mesocellular siliceous foams,with uniformly sized cells and windows”, J. Am. Chem. Soc., 121, 254-255(1999).
23 Guo, R.J., Zhang, B.Q., Sun, Y., Liu, X.F., “Lyotropic liquid crystals and applications in synthesis of nanostructured materials”, Progress in Chemistry, 19(11), 1695-1702(2007).
24 Tarasevich, Y.I., “Interaction of globular albumins with the silica surface”, Theor. Exp. Chem., 37, 98-102(2001).
25 Shirihama, H., Lyklema, J., Norde, W.J., “Comparative protein adsorption in model systems”, J. Colloid Interface Sci., 139, 177-187(1990).
26 Vinu, A., Murugesan, V., Hartmann, M., “Adsorption of lysozyme over mesoporous molecular sieves MCM-41 and SBA-15:Influence of pH and aluminum incorporation”, J. Phys. Chem. B., 108, 7323-7330(2004).
27 Fan, J., Lei, J., Wang, L.M., Yu, C.Z., Tu, B., Zhao, D.Y., “Rapid and high-capacity immobilization of enzymes based on mesoporous silicas with controlled morphologies”, Chem. Commun., 17, 2140-2141(2003).
28 Kisler, J.M., Dahler, A., Stevens, G.W., O'Connor, A.J., “Separation of biological molecules using mesoporous molecular sieves”, Microporous Mesoporous Mater., 44, 769-774(2001).
29 Katiyar, A., Ji, L., Smirniotis, P.G., Pinto, N.G., “Adsorption of bovine serum albumin and lysozyme on siliceous MCM-41”, Microporous Mesoporous Mater., 80, 311-320(2005).
30 Katiyar, A., Ji, L., Smirniotis, P.G., Pinto, N.G., “Protein adsorption on the mesoporous molecular sieve silicate SBA-15:Effects of pH and pore size”, J. Chromatogr. A., 1069, 119-126(2005).
31 Katiyar, A., Ji, L., Smirniotis, P.G., Pinto, N.G., “Adsorption of bovine serum albumin and lysozyme on silicious MCM-41”, Micropor. Mesopor. Mater., 80, 311(2005).

[1]	毛建新, 袁子卿, 严红绡, 周仁贤. 添加Sm对Pt /SBA-15催化苯完全氧化反应活性和热稳定性的影响[J]. 化工学报, 2020, 71(1): 306-313.
[2]	吕喜蕾, 阮厚航, 陈皓, 吕秀阳. 近临界乙醇中Zr-SBA-15催化糠醛一步法制备乙酰丙酸乙酯[J]. 化工学报, 2018, 69(6): 2488-2495.
[3]	胡晶晶, 赵地顺, 胡甜甜, 李静静, 翟建华. SBA-15固载酸性离子液体催化酯化反应性能[J]. 化工学报, 2016, 67(5): 1907-1914.
[4]	孙秀丽, 陈爱平, 吕慧, 何洪波, 何田田, 林启志, 李春忠. SBA-15/Ni/TiO₂/CNTs复合材料的制备及其光催化性能[J]. 化工学报, 2014, 65(9): 3718-3723.
[5]	孙秀丽, 陈爱平, 吕慧, 何洪波, 何田田, 林启志, 李春忠. SBA-15/Ni/TiO₂/CNTs复合材料的制备及其光催化性能[J]. 化工学报, 2014, 65(9): 0-0.
[6]	王兴慧, 朱桂茹, 高从堦. 短孔道介孔二氧化硅SBA-15对铀的吸附性能[J]. 化工学报, 2013, 64(7): 2480-2487.
[7]	陈立芳, 曹文荣, 苗改, 王翠花, 方云进, 漆志文. SBA-15锚定的钌杂多酸催化剂的合成及其催化正十六烷氧化[J]. 化工学报, 2013, 64(2): 547-554.
[8]	李祥珍，王晓钟，刘瑜，陈伟，黄璐. 介孔材料Al-SBA-15的合成研究进展[J]. 化工进展, 2013, 32(07): 1555-1563.
[9]	柳杨1，2，衣怀峰3，陈宇1，2，吴玉龙2，陈曾1，杨明德2，童军茂1. SBA-15的改性及催化文冠果油制备生物柴油 [J]. CIESC Journal, 2011, 30(6): 1247-.
[10]	刘少友, 陆建平, 冯庆革, 唐文华. Effect of Ethanol on Synthesis and Electrochemical Property of Mesoporous Al-doped Titanium Dioxide via Solid-state Reaction [J]. CIESC Journal, 2011, 19(4): 674-681.
[11]	胡智辉, 张东辉, 王纪孝. Direct Synthesis of Amine-functionalized Mesoporous Silica for CO₂ Adsorption[J]. CIESC Journal, 2011, 19(3): 386-390.
[12]	高婷婷, 姬广斌. Pd/CMK-3的合成及其在Suzuki-Miyaura碳-碳偶联反应中的应用 [J]. 化工学报, 2011, 62(2): 515-519.
[13]	任文玲，陈绍云，张永春，李桂民. Zn/SBA-15介孔吸附剂的制备及脱附低浓度H2S性能[J]. CIESC Journal, 2011, 30(10): 2304-.
[14]	宋伟明, 左春玲, 邓启刚. Synthesis and Characterization of Worm-shaped Tubular Lanthanum Aluminum Composite Mesoporous Materials[J]. CIESC Journal, 2011, 19(1): 70-75.
[15]	陈汇勇, 林东强, 周碧红, 吴永标, 奚红霞, 钱宇. 有机模板剂和硅酸物种的电荷效应对SBA-15介观组装结构的影响 [J]. 化工学报, 2010, 61(3): 682-690.

Effect of Pore-size of Mesoporous SBA-15 on Adsorption of Bovine Serum Albumin and Lysozyme Protein

Effect of Pore-size of Mesoporous SBA-15 on Adsorption of Bovine Serum Albumin and Lysozyme Protein

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价