Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture

CIESC Journal ›› 2011, Vol. 19 ›› Issue (1): 108-115.

Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture

喻国策^1,2, 施定基³, 蔡昭铃², 丛威², 欧阳藩²

1. Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China;
2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China;
3. Research Center of Photosynthesis, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

收稿日期:2010-04-06 修回日期:2010-04-06 出版日期:2011-02-28 发布日期:2011-02-28
通讯作者: YU Guoce, E-mail: yugc@tsinghua.edu.cn
基金资助:
Supported by a grant from the State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences

Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture

YU Guoce^1,2, SHI Dingji³, CAI Zhaoling², CONG Wei², OUYANG Fan²

1. Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China;
2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China;
3. Research Center of Photosynthesis, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Received:2010-04-06 Revised:2010-04-06 Online:2011-02-28 Published:2011-02-28
Supported by:
Supported by a grant from the State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences

摘要/Abstract

摘要： Mixotrophic growth is one potential mode for mass culture of microalgae and cyanobacteria particularly suitable for the production of high value bioactive compounds and fine chemicals.The typical heterocystous cyanobacterium Anabaena sp.PCC 7120 was grown in the presence of exogenous glucose in light.Glucose improved the cell growth evidently,the maximal specific growth rate under mixotrophic condition(0.38 d^-1)being 1.6-fold of that of photoautotrophic growth.Mixotrophy caused a variation in cellular pigment composition,increasing the content of chlorophyll a and decreasing the contents of carotenoid and phycobiliprotein relative to chlorophyll a.Fluorescence emission from photosystem Ⅱ(PSⅡ)relative to photosystem I was enhanced in mixotrophic cells,implying an increased energy distribution in PSⅡ.Glucokinase(EC 2.7.1.2)activity was further induced in the presence of glucose.The mixotrophic culture was scaled up in a 15 L airlift photobioreactor equipped with an inner and an outer light source.A modified Monod model incorporating the specific growth rate and the average light intensity in the reactor was developed to describe cell growth appropriately.The understanding of mixotrophic growth and relevant physiological features of Anabaena sp.PCC 7120 would be meaningful for cultivation and exploitation of this important cyanobacterial strain.

关键词: cyanobacteria, Anabaena sp.PCC 7120, growth features, mixotrophic culture, photobioreactor

Abstract: Mixotrophic growth is one potential mode for mass culture of microalgae and cyanobacteria particularly suitable for the production of high value bioactive compounds and fine chemicals.The typical heterocystous cyanobacterium Anabaena sp.PCC 7120 was grown in the presence of exogenous glucose in light.Glucose improved the cell growth evidently,the maximal specific growth rate under mixotrophic condition(0.38 d^-1)being 1.6-fold of that of photoautotrophic growth.Mixotrophy caused a variation in cellular pigment composition,increasing the content of chlorophyll a and decreasing the contents of carotenoid and phycobiliprotein relative to chlorophyll a.Fluorescence emission from photosystem Ⅱ(PSⅡ)relative to photosystem I was enhanced in mixotrophic cells,implying an increased energy distribution in PSⅡ.Glucokinase(EC 2.7.1.2)activity was further induced in the presence of glucose.The mixotrophic culture was scaled up in a 15 L airlift photobioreactor equipped with an inner and an outer light source.A modified Monod model incorporating the specific growth rate and the average light intensity in the reactor was developed to describe cell growth appropriately.The understanding of mixotrophic growth and relevant physiological features of Anabaena sp.PCC 7120 would be meaningful for cultivation and exploitation of this important cyanobacterial strain.

Key words: cyanobacteria, Anabaena sp.PCC 7120, growth features, mixotrophic culture, photobioreactor

喻国策, 施定基, 蔡昭铃, 丛威, 欧阳藩. Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture[J]. CIESC Journal, 2011, 19(1): 108-115.

YU Guoce, SHI Dingji, CAI Zhaoling, CONG Wei, OUYANG Fan. Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture[J]. , 2011, 19(1): 108-115.

参考文献

1 Marquez, F.J., Nishio, N., Nagai, S., Sasaki, K., “Enhancement of biomass and pigment production during growth of Spirulina platensis in mixotrophic culture”, J. Chem. Technol. Biotechnol., 62, 159-164 (1995).
2 Chen, F., “High cell density culture of microalgae in heterotrophic growth”, Trends Biotechnol., 14, 421-426 (1996).
3 Chen, F., Zhang, Y., “High cell density mixotrophic culture of Spirulina platensis on glucose for phycocyanin production using a fed-batch system”, Enzyme Microb. Technol., 20, 221-224 (1997).
4 Droop, M.R., “Heterotrophy of carbon”, In: Algal Physiology and Biochemistry, University of California Press, Berkeley and Los Angeles, 530-559 (1974).
5 Smith, A.J., “Modes of cyanobacterial carbon metabolism”, In: The Biology of Cyanobacteria, Blackwell Scientific Publications, Oxford, London, Edinburgh, Boston and Melbourne, 47-85 (1982).
6 Tuchman, N.C., “The role of heterotrophy in algae”, In: Algal Ecology, Academic Press, San Diego, 299-319 (1996).
7 Lee, Y.K., Ding, S.Y., Hoe, C.H., Low, C.S., “Mixotrophic growth of Chlorella sorokiniana in outdoor enclosed photobioreactor”, J. Appl. Phycol., 8, 163-169 (1996).
8 Martinez, M.E., Camacho, F., Jimenez, J.M., Espinola, J.B., “Influence of light intensity on the kinetic and yield parameters of Chlorella pyrenoidosa mixotrophic growth”, Process Biochem., 32, 93-98 (1997).
9 Ip, P.F., Wong, K.H., Chen, F., “Enhanced production of astaxanthin by the green microalga Chlorella zofingiensis in mixotrophic culture”, Process Biochem., 39, 1761-1766 (2004).
10 Liang, Y., Sarkany, N., Cui, Y., “Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions”, Biotechnol. Lett., 31, 1043-1049 (2009).
11 Kobayashi, M., Kakizono, T., Yamaguchi, K., Nishio, N., Nagai, S., “Growth and astaxanthin formation of Haematococcus pluvialis in heterotrophic and mixotrophic conditions”, J. Ferment. Bioeng., 74,17-20 (1992).
12 Zhang, X.W., Gong, X.D., Chen, F., “Kinetic models for astaxanthin production by high cell density mixotrophic culture of the microalga Haematococcus pluvialis”, J. Ind. Microbiol. Biotechnol., 23,691-696 (1999).
13 Jeon, Y.C., Cho, C.W., Yun, Y.S., “Combined effects of light intensity and acetate concentration on the growth of unicellular microalga Haematococcus pluvialis”, Enzyme Microb. Technol., 39, 490-495 (2006).
14 Shamala, T.R., Drawert, F., Leupold, G., “Studies on Scenedesmus acutus growth. I. Effect of autotrophic and mixotrophic conditions on the growth of Scenedesmus acutus”, Biotechnol. Bioeng., 24,1287-1299 (1982).
15 Combres, C., Laliberte, G., Reyssac, J.S., Delanoue, J., “Effect of acetate on growth and ammonium uptake in the microalga Scenedesmus obliquus”, Physiol. Plant., 91, 729-734 (1994).
16 Xie, J., Zhang, Y., Li, Y., Wang, Y., “Mixotrophic cultivation of Platymonas subcordiformis”, J. Appl. Phycol., 13, 343-347 (2001).
17 Rippka, R., “Photoheterotrophy and chemoheterotrophy among unicellular blue-green algae”, Arch. Microbiol., 87, 93-98 (1972).
18 Stal, L.J., Moezelaar, R., “Fermentation in cyanobacteria”, FEMS Microbiol. Rev., 21, 179-211 (1997).
19 Marquez, F.J., Sasaki, K., Kakizono, T., Nishio, N., Nagai, S., “Growth characteristics of Spirulina platensis in mixotrophic and heterotrophic conditions”, J. Ferment. Bioeng., 76, 408-410 (1993).
20 Chen, F., Zhang, Y., Guo, S., “Growth and phycocyanin formation of Spirulina platensis in photoheterotrophic culture”, Biotechnol. Lett.,18, 603-608 (1996).
21 Chen, T., Zheng, W., Yang, F., Bai, Y., Wong, Y.S., “Mixotrophic culture of high selenium-enriched Spirulina platensis on acetate and the enhanced production of photosynthetic pigments”, Enzyme Microb. Technol., 39, 103-107 (2006).
22 Wang, Y., Li, Y., Shi, D., Shen, G., Ru, B., Zhang, S., “Characteristics of mixotrophic growth of Synechocystis sp. in an enclosed photobioreactor”, Biotechnol. Lett., 24, 1593-1597 (2002).
23 Yu, H., Jia, S., Dai, Y., “Growth characteristics of the cyanobacterium Nostoc flagelliforme in photoautotrophic, mixotrophic and heterotrophic cultivation”, J. Appl. Phycol., 21, 127-133 (2009).
24 Haury, J.F., Spiller, H., “Fructose uptake and influence on growth of and nitrogen fixation by Anabaena variabilis”, J. Bacteriol., 147,227-235 (1981).
25 Rozen, A., Arad, H., Schonfeld, M., Tel-Or, E., “Fructose supports glycogen accumulation, heterocysts differentiation, N₂ fixation and growth of the isolated cyanobiont Anabaena azollae”, Arch. Microbiol., 145, 187-190 (1986).
26 Rozen, A., Schonfeld, M., Tel-Or, E., “Fructose-enhanced development and growth of the N₂ -fixing cyanobiont Anabaena azollae”, Z. Naturforsch., 43c, 408-412 (1988).
27 Valiente, E.F., Nieva, M., Avendano, M.C., Maeso, E.S., “Uptake and utilization of fructose by Anabaena variabilis ATCC 29413. Effect on respiration and photosynthesis”, Plant Cell Physiol., 33,307-313 (1992).
28 Vonshak, A., Cheung, S.M., Chen, F., “Mixotrophic growth modifies the response of Spirulina (Arthrospira) platensis (cyanobacteria) cells to light”, J. Phycol., 36, 675-679 (2000).
29 Rippka, R., Deruelles, J., Waterbury, J.B., Herdman, M., Stanier, R.Y., “Generic assignments, strain histories and properties of pure cultures of cyanobacteria”, J. Gen. Microbiol., 111, 1-61 (1979).
30 Bryant, D.A., The Molecular Biology of Cyanobacteria, Kluwer Academic Publishers, The Netherlands (1994).
31 Kaneko, T., Nakamura, Y., Wolk, C.P., Kuritz, T., Sasamoto, S., Watanabe, A., Iriguchi, M., Ishikawa, A., Kawashima, K., Kimura, T., Kishida, Y., Kohara, M., Matsumoto, M., Matsuno, A., Muraki, A., Nakazaki, N., Shimpo, S., Sugimoto, M., Takazawa, M., Yamada, M., Yasuda, M., Tabata, S., “Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC7120”, DNA Res., 8, 227-253 (2001).
32 Archer, S.D., McDonald, K.A., Jackman, A.P., “Effect of light irradiance on the production of sulfolipids from Anabaena 7120 in a fed-batch photobioreactor”, Appl. Biochem. Biotechnol., 67, 139-152 (1997).
33 Morales, E., Rodriguez, M., Garcia, D., Loreto, C., Marco, E., “Effect of pH and CO₂ on growth, pigments and exopolysaccharides production from cyanobacteria Anabaena sp. PCC 7120”, Interciencia, 27, 373-378 (2002).
34 Ren, L., Shi, D.J., Dai, J.X., Ru, B.G., “Expression of the mouse metallothionein-I gene conferring cadmium resistance in a transgenic cyanobacterium”, FEMS Microbiol. Lett., 158, 127-132 (1998).
35 Liu, F.L., Zhang, H.B., Shi, D.J., Shang, Z.D., Lin, C., Shao, N., Peng, G.H., Zhang, X.Y., Zhang, H.X., Wu, J.Y., Wang, J., Xu, X.D., Jiang, Y.H., Zhong, Z.P., Zhao, S.J., Wu, M., Zeng, C.K., “Construction of shuttle, expression vector of human tumor necrosis factor alpha (hTNF-α) gene and its expression in a cyanobacterium, Anabaena sp. PCC7120”, Sci. China (Series C), 42, 25-33 (1999).
36 Castenholz, R.W., “Culturing methods for cyanobacteria”, In: Methods in Enzymology, Vol. 167, Cyanobacteria, Academic Press, San Diego, 68-93 (1988).
37 Williams, J.G.K., “Construction of specific mutations in photosystem II photosynthetic reaction center by genetic engineering methods in Synechocystis 6803”, In: Methods in Enzymology, Vol. 167, Cyanobacteria, Academic Press, San Diego, 766-778 (1988).
38 Slein, M.W., Cori, G.T., Cori, C.F., “A comparative study of hexokinase from yeast and animal tissues”, J. Biol. Chem., 186,763-780 (1950).
39 Pearce, J., Carr, N.G., “The incorporation and metabolism of glucose by Anabaena variabilis”, J. Gen. Microbiol., 54, 451-462 (1969).
40 Markwell, M.A.K., Haas, S.M., Bieber, L.L., Tolbert, N.E., “A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples”, Anal. Biochem., 87,206-210 (1978).
41 Hirata, S., Taya, M., Tone, S., “Characterization of Chlorella cell cultures in batch and continuous operations under a photoautotrophic condition”, J. Chem. Eng. Japan, 29, 953-959 (1996).
42 Scherer, S., “Do photosynthetic and respiratory electron transport chains share redox proteins”, Trends Biochem. Sci., 15, 458-462 (1990).
43 Knowles, V.L., Plaxton, W.C., “From genome to enzyme: analysis of key glycolytic and oxidative pentose-phosphate pathway enzymes in the cyanobacterium Synechocystis sp. PCC 6803”, Plant Cell Physiol., 44, 758-763 (2003).
44 Sundaram, S., Karakaya, H., Scanlan, D.J., Mann, N.H., “Multiple oligomeric forms of glucose-6-phosphate dehydrogenase in cyanobacteria and the role of OpcA in the assembly process”, Microbiology, 144, 1549-1556 (1998).

Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture

Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture

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