Abstract:

Eleven different macroporous adsorption resins were studied with respect to the static adsorption capacity and desorption ratio of vinblastine, vindoline and catharanthine.The best resin was AB-8 and the adsorption capacities of vindoline, catharanthine and vinblastine were 365.8, 254.2 and 24.8 mg·ml^-1, respectively.The optimum parameters of separation process by AB-8 resin were as follows: extraction solvent was sulfate dilution; pH value of the extraction was adjusted to 8 by ammonia; elute solvents were 20% alcohol to remove polar components, 50% alcohol (pH=4) to obtain the mono-indole alkaloids (vindoline and catharanthine) at 30℃ and 90% alcohol to obtain the dimeric-indole alkaloid (vinblastine).The results showed that the content of mono-indole alkaloids was above 50% and the purity of vinblastine was above 60%.