野油菜黄单胞菌反枝苋致病菌毒素的分离和结构分析

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野油菜黄单胞菌反枝苋致病菌毒素的分离和结构分析

李明智^1,2; 徐凌¹; 孙自伶¹; 李永泉¹

¹ College of Life Science, Zhejiang University, Hangzhou 310058, China
² Research Institute of Subtropical Forestry, China Academy of Forestry, Fuyang 311400, China

收稿日期:2007-01-05 修回日期:1900-01-01 出版日期:2007-10-28 发布日期:2007-10-28
通讯作者: 李明智

Isolation and characterization of a phytotoxin from Xanthomonas campestris pv. retroflexus

LI Mingzhi^1,2; XU Ling¹; SUN Ziling¹; LI Yongquan¹

¹ College of Life Science, Zhejiang University, Hangzhou 310058, China
² Research Institute of Subtropical Forestry, China Academy of Forestry, Fuyang 311400, China

Received:2007-01-05 Revised:1900-01-01 Online:2007-10-28 Published:2007-10-28
Contact: LI Mingzhi

摘要/Abstract

摘要： A phytotoxin from Xanthomonas campestris pv. retroflexus was isolated using a chromatographer and HPLC, and the components were identified to be a mixture of minor molecular compounds including organic acids and cyclo-(proline-phenylalanine). The greenhouse cultivation test was used to determine the influence of the isolated fractions on the growth of target weed redroot pigweed (Amaranthus retroflexus L). The experimental results demonstrated that the cyclo-(Pro-Phe) had the weed inhibit activity obviously on dicotyledonous weed and the mixture with six organic acids showed stronger bioactivity. Further, greenhouse and field test were processed, and the test showed that the use of the toxin appeared to have the potential to be developed further as a bioherbicide system to control weedy grasses.

关键词: Xanthomonas campestris pv. retroflexus;phytotoxin;isolation;identification;minor molecular compounds;cyclo-(Pro-Phe);redroot pigweed;bioherbicide, bioherbicide

Abstract: A phytotoxin from Xanthomonas campestris pv. retroflexus was isolated using a chromatographer and HPLC, and the components were identified to be a mixture of minor molecular compounds including organic acids and cyclo-(proline-phenylalanine). The greenhouse cultivation test was used to determine the influence of the isolated fractions on the growth of target weed redroot pigweed (Amaranthus retroflexus L). The experimental results demonstrated that the cyclo-(Pro-Phe) had the weed inhibit activity obviously on dicotyledonous weed and the mixture with six organic acids showed stronger bioactivity. Further, greenhouse and field test were processed, and the test showed that the use of the toxin appeared to have the potential to be developed further as a bioherbicide system to control weedy grasses.

Key words: Xanthomonas campestris pv. retroflexus, phytotoxin, isolation, identification, minor molecular compounds, cyclo-(Pro-Phe)

李明智, 徐凌, 孙自伶, 李永泉. 野油菜黄单胞菌反枝苋致病菌毒素的分离和结构分析[J]. CIESC Journal.

LI Mingzhi, XU Ling, SUN Ziling, LI Yongquan. Isolation and characterization of a phytotoxin from Xanthomonas campestris pv. retroflexus[J]. .

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野油菜黄单胞菌反枝苋致病菌毒素的分离和结构分析

Isolation and characterization of a phytotoxin from Xanthomonas campestris pv. retroflexus

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