响应面法优化工程菌产细胞色素P450 BM-3的发酵条件
CIESC Journal
• 生物化学工程、制药、食品和天然产物加工 • 上一篇 下一篇
陆燕;梅乐和;陆悦飞;盛清;姚善泾
浙江大学化学工程与生物工程学系,浙江 杭州 310027;浙江理工大学生命科学学院,浙江 杭州 310037
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Optimization of P450 BM-3 activity produced by gene engineering strain by response surface methodology
LU Yan;MEI Lehe;LU Yuefei;SHENG Qing;YAO Shanjing
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摘要: 采用快速有效的数学统计方法对工程菌产细胞色素P450 BM-3的发酵条件进行了优化.首先利用Plackett-Burman设计从众多影响产P450 BM-3的因素中筛选出影响较大的4个因素:FeCl3含量、诱导时机、诱导时间和接种量.在此基础上再利用响应面法中的杂合设计进行优化,通过拟合得到响应曲面函数,获得了最佳的实验条件.在该实验条件下,P450 BM-3酶活从37.6×10-3U8226;ml-1提高到57.9×10-3U8226;ml-1.
Abstract: Factorial design and response surface techniques were used to design and optimize the process to increase P450 BM-3 activity produced by gene engineering strain.Initially, Plackett-Burman design was used to evaluate the process variables which were relevant to the production of P450 BM-3.Four statistically significant parameters for response were selected and utilized in order to optimize the process.With the 416C model of hybrid design, response surfaces were generated, and P450 BM-3 activity was increased to 57.9×10-3Uml-1 from 37.6×10-3Uml-1 by the best combination of the physicochemical parameters at optimal levels of 0.12 mgL-1 FeCl3, inoculum volume of 2.1%, induction at OD578=1.1, and 6 h of induction.
陆燕;梅乐和;陆悦飞;盛清;姚善泾.
LU Yan;MEI Lehe;LU Yuefei;SHENG Qing;YAO Shanjing.
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https://hgxb.cip.com.cn/CN/Y2006/V57/I5/1187