Abstract: The disruption kinetics of a microbial cell was studied by using a horizontal bead mill (Dyno -mill). A mechanism was put forward on the basis of experimental results and prior work. It was composed of the release of intracellular products,and their solubilization and inactivation during the cell disruption process. A new model of disruption kinetics was established, which was used to regress the experimental data and calculate the relevant parameters. The new kinetics equation could better explain the deactivation phenomena of intracellular products.