Abstract:

The proposition and development of the newly emerging nano-cryopreservation of biological materials are reviewed and summarized. Attentions are focused on the elucidation of the freezing response of biological materials with nano-particle solution，the effects of nano-particles on cryo-protectants and the nucleation mechanism of nano-particle solution. Technical strategies through introduction of nan-particles to efficiently improve the function of cryo-protectant are interpreted. The latest research status in the field is analyzed. Future directions worthy of pursuing are discussed.