Abstract: 9-Fluorenyl methoxy carbonyl(Fmoc)solid-phase synthesis of Fusukang was studied firstly, including stepwise solid-phase peptide synthesis(SPPS) and solid-phase convergent synthesis.The SPPS methodology utilized DMF as the coupling solvent, HBTU/HOBt/DIEA as the coupling reagent, Wang resin as solid supporter, TFA/p-methyl phenol/EDT/H2O(97∶0.25∶0.25∶2.5) as the cleavage condition, with 2-fold equivalent molar excess of protected amino acid.In the Fmoc solid-phase convergent synthesis of Fusukang, the effects of convergent protocol, coupling solvents, and coupling reagents on the yield of Fusukang were systemically studied, and the protocol of condensation process was optimized, i.e.using the sequential condensation of four protected peptide segments(1—10，11—20，21—25，26—36) with 1.5-fold equivalent molar excess of protected peptide segments in solvent; HATU/HOAt/DIEA (4-fold equivalent) as the coupling reagent; mixture DMF/DCM(1∶1,vol) as the coupling solvent; TFA/p-methyl phenol/EDT/H2O (97∶0.25∶0.25∶2.5) as the cleavage condition, washing resin 5 times with DCM before and after each reaction.The yield of crude Fusukang was 67.31% , and the purity was 38.75%.