Abstract: Using polysulfone (PSF) hollow fiber membrane as the supporting membrane, chiral (D-tryptophane) molecular imprinted hollow fiber composite membrane (CMIHFCM) was fabricated by the surface photo-polymerization method and the recognition ability of CMIHFCM for D,L-tryptophane was studied.After surface polymerization, the external surface of CMIHFCM had a complex network layer with a thickness of 3 μm on the supporting membrane.The experimental results showed that CMIHFCM had the recognition ability for the template D-tryptophane.The D- tryptophane solution could be condensed more significantly by the molecular imprinted membrane than L-tryptophane,and the separation factor (α) of D- tryptophane (D-Trp) and L-tryptophane (L-Trp) could be 5.0.However, the recognition for D-tryptophane by the non-molecular imprinted membrane was weaker.When the concentration of initiator was 1.0%, CMIHFCM with good recognition ability for D-Trp was obtained.