CIESC Journal

• 生物化学工程、制药、食品和天然产物加工 • 上一篇    下一篇

数字化无阀微泵在细胞输送中的应用实验

沙菁洁 侯丽雅 章维一 田桂中   

  1. 南京理工大学机械工程学院微系统研究室 南京理工大学机械学院206教研室 南京理工大学机械学院170幢505室
  • 出版日期:2007-02-05 发布日期:2007-02-05

Cell feeding experiment by digital valveless micro-pump

  

  • Online:2007-02-05 Published:2007-02-05

摘要: 显微细胞注射中,一般操作是细胞在培养液中,吸持针在培养液中寻找细胞,气压吸持住细胞,然后注射,但是该方法存在已注射和未注射的细胞容易混淆和注射效率很低等问题。本文介绍应用数字化无阀微泵实现定点连续输送细胞,将会提高显微注射效率和成功率。

Abstract: During micro injection into cells, the usual manipulation is that a holding needle finds and holds the cells into which will be injected,in the nutrient medium.But there are some shortcomings in this method, just as confusion about whether injection is made or not, poor injection efficiency and othersThis paper presents cell pointing feeding by a digital valveless micro-pump, which can improve the success rate of the micro injectionThis micro-pump draws the nurture fluid enwrapping cells into the inlet by capillary force, then ejects them based on the digital micro fluidic technologyThere exists a pressure gap between inlet and outlet,and the inlet forms the lock.Thus, cells in this micro-pump flows in one direction The pumped cells would be injected into one by one efficiently.