Abstract: The formulas describing the dependence of blood perfusion rate on temperature were set up for the whole temperature range from hypothermia to hyperthermia.The influence of the temperature dependent blood perfusion rate and metabolic heat generation on the transient thermal field and thermal gradient field were studied by using finite element analysis.The enthalpy method was used to solve the thermophysical nonlinear and multidimensional phase change problem in cryosurgery of the target tissue, and the tissue was treated as non-ideal materials freezing over a temperature range.Theoretical simulation indicated that without full consideration of the thermal dependence of blood perfusion rate and metabolic heat, the transient temperature of the typical point or the temperature along the typical path inside the tissue would both be overestimated, the temperature difference even reached about 5℃;the thermal gradients of the typical points inside the tissue or on the cryoprobe surface were also overestimated.Besides, the thermal gradient of the typical point on the cryoprobe surface was much larger than that of the point inside the tissue, and this might have certain relation to the often appearing cracks or ruptures inside the tissue closely tied to the cryoprobe surface during cryosurgical process.This study suggested that the thermal dependence of blood perfusion rate and metabolic heat generation should be fully incorporated into the analysis of the heat transfer problem in cryosurgery and should be fully considered for the design/optimization of the cryosurgical procedure and cryoprobe.