CIESC Journal

• 能源和环境工程 • 上一篇    下一篇

强化生物除磷工艺微生物种群结构分析

李伟光1,2,田文德1,康晓荣1,张卉1,郭旋1   

  1. 1哈尔滨工业大学市政与环境工程学院,黑龙江 哈尔滨 150090; 2城市水资源开发利用(北方)国家工程研究中心,黑龙江 哈尔滨 150090
  • 出版日期:2011-12-05 发布日期:2011-12-05

Analysis of microbial community structure in enhanced biological dephosphorization

  • <<,TIAN Wende1,KANG Xiaorong1, ZHANG Hui1, GUO Xuan1   

    • Online:2011-12-05 Published:2011-12-05
  • Abstract:

    In order to study the microbial community structure and diversity as well as the performance of phosphorus removal associated with microbial community change, the fingerprint profile technique of PCR amplified 16S rRNA and denaturing gradient gel electrophoresis (DGGEwere applied to analyse the microbial communities in Bi-Bio-Selective simultaneous Phosphorus and Nitrogen removal (BBSPNprocess.After the characteristic bands eluted from gel were further recovered, cloned (reamplifiedand sequenced, the sequences were compared with those in GenBank database and used for homology analysis, and the phylogenetic trees were constructed.The sequence of DGGE bands obtained showed that there is a good dynamic correlation between microbial community variation and its removal performance of process.TP removal efficiency of BBSPN process was lower when α-proteobacteria was the dominant species, which mean α-proteobacteria was not favorable for enhanced biological phosphorus removal; whereas when Bacteroidetes was the dominant species, TP removal efficiency and denitrifying dephosphorization were better and stable, which should attribute to Uncultured Sphingobacteriaceae.So, the Uncultured Sphingobacteriaceae was like to be one of the denitrifying phosphate accumulating organisms(DPAOsin this process.Moreover, Acinetobacter,TM7 and Uncultured Trichococcus are also beneficial for phosphorus and nitrogen removal of BBSNP process.

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