Abstract: Lipase is an important industrial enzyme. This review summarizes the application of Pseudomonas lipases in resolution of chiral alcohols and esters which are important intermediates for medical and fine chemicals. Besides，mechanism for the enantioselective performance of Pseudomonas lipase by molecular modeling，and the method for enantioselective enhancement by directed evolution are discussed. Furthermore，due to the nature of organic solvent tolerance，Pseudomonas lipases can be used in non-aqueous biocatalysis. With the interdisciplinary development of biology and chemistry，Pseudomonas lipases will have great potential in development and application in the future.