化工学报 ›› 2020, Vol. 71 ›› Issue (9): 4255-4259.DOI: 10.11949/0438-1157.20200527

• 生物化学工程与技术 • 上一篇    下一篇

荧光假单胞杆菌磷脂酶B界面催化分子机制初探

赵依威(),姜芳燕,李春,戴大章()   

  1. 北京理工大学化学与化工学院,北京 100081
  • 收稿日期:2020-05-08 修回日期:2020-07-18 出版日期:2020-09-05 发布日期:2020-09-05
  • 通讯作者: 戴大章
  • 作者简介:赵依威(1995—),男,硕士研究生,zhaoyiwei7139@163.com
  • 基金资助:
    国家自然科学基金项目(21276025)

Interfacial catalytic mechanism of Pseudomonas fluorescens phospholipase B

Yiwei ZHAO(),Fangyan JIANG,Chun LI,Dazhang DAI()   

  1. School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China
  • Received:2020-05-08 Revised:2020-07-18 Online:2020-09-05 Published:2020-09-05
  • Contact: Dazhang DAI

摘要:

基于荧光假单胞杆菌磷脂酶B(Pf-PLB)一级序列,分析了Pf-PLB的相关理化性质及其二级结构,并采用同源建模的方法对Pf-PLB的三级结构进行了预测;基于其预测结构,提出“掀盖进入,侧向离去”假说模型,初步探讨了Pf-PLB界面催化的可能机制;采用定点突变的方法对Pf-PLB的结构进行改造,结合突变体Pf-PLB的催化特性对所提出的机制模型进行了验证。

关键词: 磷脂酶B, 结构预测, 催化机制

Abstract:

The physicochemical property and secondary structure of Pseudomonas fluorescens phospholipase B(Pf-PLB) were analyzed base on its primary structure, and the tertiary structure of Pf-PLB was predicated. A “lid-in, side-out” hypothesis model was put forward to illuminate the possible interfacial catalytic mechanism of Pf-PLB. The structure of Pf-PLB was modified by site-directed mutagenesis, and the proposed mechanism model was verified by combining with the catalytic properties of mutant Pf-PLB.

Key words: phospholipase B, structure prediction, catalytic mechanism

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