关键词: 西酞普兰, 手性色谱分离, 吸附等温线

Abstract: The enantioseparation of citalopram enantiomers was investigated using high performance liquid chromatography to optimize chromatographic conditions.Chiral stationary phases（CSPs)including derivatized polysaccharide CSPs, crosslinked diallyltartrinine amide CSPs and cyclodextrin-based CPSs were used as stationary phases.n-Hexane with a small amount of diethylamine（0.1%,volume)modified with methanol, ethanol and 2-propanol were used as mobile phase.The influence on retention factor, enantioselectivity, resolution, theoretical plates and asymmetry factor were studied.The effect of temperature was also investigated on the most favorable CSP and mobile phase.Chiralpak AD-H（derivatized polysaccharide CSP)and 2-propanol were proved to be the most favorable CSP and modifier, and 5%（vol)of 2-propanol was the preferred concentration.It was found that among the temperature range of 293.15—308.15 K, higher temperature was favorable to the enantioseparation.The adsorption isotherms of citalopram enantiomers were determined by frontal analysis chromatography under the optimized chromatographic conditions.The isotherms showed convex shape and well fitted by the Langmuir adsorption isotherm model.This work provides the basic data for separation of citalopram on SMB.

Key words: citalopram, chiral chromatography, adsorption equilibrium