嗜麦芽窄食单胞菌处理苯并［a］芘-铜复合污染过程中细胞表面特性的变化

doi:10.3969/j.issn.0438-1157.2012.05.038

化工学报 ›› 2012, Vol. 63 ›› Issue (5): 1592-1598.DOI: 10.3969/j.issn.0438-1157.2012.05.038

嗜麦芽窄食单胞菌处理苯并［a］芘-铜复合污染过程中细胞表面特性的变化

陈烁娜1,2，尹华1,2，叶锦韶1,2，彭辉2，张娜1,2，何宝燕1,2

1暨南大学环境工程系；2广东省高校毒害性污染物防治与生物修复重点实验室

收稿日期:2011-08-08 修回日期:2011-10-25 出版日期:2012-05-05 发布日期:2012-05-05
通讯作者: 尹华

Change of cell surface features of Stenotrophomonas maltophilia in treating benzo［a］pyrene-copper combined pollution

CHEN Shuona1,2，YIN Hua1,2，YE Jinshao1,2，PENG Hui2，ZHANG Na1,2，HE Baoyan1,2

1 Department of Environmental Engineering，Jinan University;2Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes

Received:2011-08-08 Revised:2011-10-25 Online:2012-05-05 Published:2012-05-05

摘要/Abstract

摘要： 利用嗜麦芽窄食单胞菌(Stenotrophomonas maltophilia)对BaP-Cu进行降解/吸附实验，考察菌体处理污染物过程中细胞表面特性的变化。结果表明，S.maltophilia细胞表面具有较高的疏水性。从4 h到72 h，不同污染体系中S.maltophilia的CSH均维持在83.3%～95.6%，之后随着时间的推移，CSH开始逐渐降低。污染物可以使S.maltophilia细胞质膜的通透性增加。TEM检测结果证实处理污染物之后，菌体质膜通透性改变，破裂并出现了“孔洞”，导致细胞质外流，但是细胞壁依然维持细胞形状而没有完全裂解。处理了复合污染物的菌体，到第5 d细胞严重变形，周质空间变大，细胞质外泄严重，而且原核分解并逐渐消失，细胞活性降低，并逐渐丧失对重金属的吸附能力。

关键词: 嗜麦芽窄食单胞菌, 复合污染, 细胞疏水性, 质膜通透性

Abstract: Change of cell surface features of Stenotrophomonas maltophilia in treating BaP and Cu²⁺ composite pollutants was studied.The results indicated that S.maltophilia was of higher cell surface hydrophobicity(CSH).It could keep within 83.3%—95.6% in treating different pollutant systems for 4—72 h，and then the CSH decreased with increase of operation time.BaP and Cu²⁺ can make permeability of the cell membrane increase.The morphological features of S.maltophilia observed by TEM showed that cell membrane ruptured and appeared holes on it after treating pollutants，leading to permeability change of cell membrane and outflow of the cytoplasm，but cell wall still kept the cell in original shape without complete splitting.However，after contacting combined pollutants for 5 day，bacterial cells were out of shape seriously，periplasmic space enlarged，and cytoplasm leaked，nucleus broke down and gradually disappeared，resulting in decrease of cell activity and in gradual loss of S.maltophilia’s ability adsorbing heavy metals.

Key words: Stenotrophomonas maltophilia, combined pollution;cell surface hydrophobicity;membrane permeability

陈烁娜，尹华，叶锦韶，彭辉，张娜，何宝燕. 嗜麦芽窄食单胞菌处理苯并［a］芘-铜复合污染过程中细胞表面特性的变化[J]. 化工学报, 2012, 63(5): 1592-1598.

CHEN Shuona，YIN Hua，YE Jinshao，PENG Hui，ZHANG Na，HE Baoyan. Change of cell surface features of Stenotrophomonas maltophilia in treating benzo［a］pyrene-copper combined pollution[J]. CIESC Journal, 2012, 63(5): 1592-1598.

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嗜麦芽窄食单胞菌处理苯并［a］芘-铜复合污染过程中细胞表面特性的变化

Change of cell surface features of Stenotrophomonas maltophilia in treating benzo［a］pyrene-copper combined pollution

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