用硫酸铝作絮凝剂从发酵滤液中萃取青霉素时破乳效果的研究

CIESC Journal

用硫酸铝作絮凝剂从发酵滤液中萃取青霉素时破乳效果的研究

杨智发,于淑秋,陈家镛,刘守信,王建新

中国科学院化工冶金研究所,中国科学院化工冶金研究所,中国科学院化工冶金研究所,华北制药厂,华北制药厂北京100080 ,北京100080 ,北京100080 ,石家庄050015 ,石家庄050015

出版日期:1991-12-25 发布日期:1991-12-25

Demulsification by Aluminium Sulfate Flocculant During Extraction of Penicillin G from the Filtrate of Fermentation Broth

Yang Zhifa, Yu Shuqiu Liu Shouxin and Wang Jianxin and Chen Jiayong (Institute of Chemical Metallurgy, Academia Sinica, Beijing 100089) (North China Pharmaceutical Corporation, Shijiazhuang 050015)

Online:1991-12-25 Published:1991-12-25

摘要/Abstract

摘要： 本文应用铝离子水溶液的化学特点及蛋白质在溶液中结构变化的特征,研究了用硫酸铝作为青霉素萃取工艺中的破乳剂的作用规律及操作条件.中、小试结果表明,先将硫酸铝溶液与滤液混合30秒,并使混合液的pH值>4.5,萃取平衡的pH值>3.0,0.1～0.3%(mass/v)的硫酸铝即可有较好的破乳效果,但需严格控制溶液的pH值.

Abstract: On the basis of characteristics of Al(III)and proteins in aqueous solutions, the demulsification effect and operating conditions during extraction of penicillin G from the filtrate of fermentation broth with aluminium sulfate as demulsifier have been studied. If aluminium sulfate was premixed with the filtrate for 30 seconds with pH>4.5 before extraction and then kept at pH> 3.0, the demulsification ability of 0.1-0.3% (wt/v) aluminium sulfate was as good as that of alkyl trimethyl ammonium bromides with the pH strictly controlled at the required value. The quality of penicillin G product with ala-minum sulfate as demulsifier was also found to be good.

杨智发,于淑秋,陈家镛,刘守信,王建新. 用硫酸铝作絮凝剂从发酵滤液中萃取青霉素时破乳效果的研究 [J]. CIESC Journal.

Yang Zhifa, Yu Shuqiu Liu Shouxin and Wang Jianxin and Chen Jiayong (Institute of Chemical Metallurgy, Academia Sinica, Beijing 100089) (North China Pharmaceutical Corporation, Shijiazhuang 050015). Demulsification by Aluminium Sulfate Flocculant During Extraction of Penicillin G from the Filtrate of Fermentation Broth[J]. .