圆筒式离心萃取器用于PEG/盐体系萃取提纯α-淀粉酶

CIESC Journal

圆筒式离心萃取器用于PEG/盐体系萃取提纯α-淀粉酶

冯幼琴,梅乐和,朱自强,陈孝国

浙江大学化工系,浙江大学化工系,浙江大学化工系,浙江大学化工系杭州210027 ,杭州210027 ,杭州210027 ,杭州210027

出版日期:1992-10-25 发布日期:1992-10-25

CYLINDRICAL CENTRIFUGAL EXTRACTOR FOR EXTRACTION PURIFICATION OF α-AMYLASE IN AQUEOUS TWO-PHASE SYSTEMS

Fung Youqin, Mei Lehe, Zhu Ziqiang and Chen Xiaoguo (Department of Chemical Engineering, Zhejiang University, Hangzhou 310027)

Online:1992-10-25 Published:1992-10-25

摘要/Abstract

摘要： 根据双水相体系的特点,选择了Ф20(环)圆筒式离心萃取器进行a-淀粉酶的双水相体系萃取实验,考察了萃取器的水力学特性,确定了萃取操作范围,并考察了萃取器转鼓转速、总流量对体系传质特性、a-淀粉酶失活和收率的影响.结果表明,在转速3380～3800r/min、流比0.2～0.5、总流量25～50ml/min范围内,萃取器的轻相夹带量可在10%以内,重相夹带量趋近于零,a-淀粉酶的失活可以忽略不计,萃取效率和收率分别达90%和75%以上.

Abstract: The technical aspects of a new and powerful separation technique, extractive purification by aqueous two-phase systems were studied in detail. According to the characteristics of aqueous two phase systems, a φ20 cylindrical centrifugal extractor was selected as the equipment for extractive purification of α-amylase. The hydraulic characteristics of the installation and the effects of speed and total throughput on the yield and Murphree efficiency of α-amylase extraction were investigated. A speed of 3380-3800r/min, a flow ratio of 0.2-0.5 and a total throughput of 25-50ml/min were found as the optimal operation range for while 90% of phases purity, 75% yield and 90% Murphree efficiency for a-amylase could be obtained.

冯幼琴,梅乐和,朱自强,陈孝国. 圆筒式离心萃取器用于PEG/盐体系萃取提纯α-淀粉酶 [J]. CIESC Journal.

Fung Youqin, Mei Lehe, Zhu Ziqiang and Chen Xiaoguo (Department of Chemical Engineering, Zhejiang University, Hangzhou 310027). CYLINDRICAL CENTRIFUGAL EXTRACTOR FOR EXTRACTION PURIFICATION OF α-AMYLASE IN AQUEOUS TWO-PHASE SYSTEMS[J]. .