EXTRACTION OF INTERFERON-γ USING PEG/K_3PO_4 AQUEOUS TWO-PHASE SYSTEM

CIESC Journal

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EXTRACTION OF INTERFERON-γ USING PEG/K_3PO_4 AQUEOUS TWO-PHASE SYSTEM

沈忠耀; 丛梅; 杨基础

Department of Chemical Engineering, Tsinghua University, Beijing 100084, China

收稿日期:1991-10-26 修回日期:1992-06-13 出版日期:1993-09-28 发布日期:1993-09-28
通讯作者: 沈忠耀

EXTRACTION OF INTERFERON-γ USING PEG/K_3PO_4 AQUEOUS TWO-PHASE SYSTEM

SHEN Zhongyao; CONG Mei; YANG Jichu

Department of Chemical Engineering, Tsinghua University, Beijing 100084, China

Received:1991-10-26 Revised:1992-06-13 Online:1993-09-28 Published:1993-09-28
Contact: SHEN Zhongyao

摘要/Abstract

摘要： The aqueous two-phase system of PEG-4000/K_3PO_4 is selected to extract interferon-γ(γ-IFN)from the broth of homogenized E.coil cells,which were recombined genetically and werefermented in high expressivity.The effects of pH value and the concentrations of PEG and phos-phate on the partition behavious of γ-IFN and contaminating proteins have been investigated.Theresults show that the pH value is the most influential factor effecting the extraction yield of γ-IFN.Under a suitable condition,an effective separation with high yield of γ-IFN can be achieved.

关键词: aqueous two-phase extraction;interferon-γ

Abstract: The aqueous two-phase system of PEG-4000/K_3PO_4 is selected to extract interferon-γ(γ-IFN)from the broth of homogenized E.coil cells,which were recombined genetically and werefermented in high expressivity.The effects of pH value and the concentrations of PEG and phos-phate on the partition behavious of γ-IFN and contaminating proteins have been investigated.Theresults show that the pH value is the most influential factor effecting the extraction yield of γ-IFN.Under a suitable condition,an effective separation with high yield of γ-IFN can be achieved.

Key words: aqueous two-phase extraction, interferon-γ

沈忠耀, 丛梅, 杨基础. EXTRACTION OF INTERFERON-γ USING PEG/K_3PO_4 AQUEOUS TWO-PHASE SYSTEM[J]. CIESC Journal.

SHEN Zhongyao, CONG Mei, YANG Jichu. EXTRACTION OF INTERFERON-γ USING PEG/K_3PO_4 AQUEOUS TWO-PHASE SYSTEM[J]. .

EXTRACTION OF INTERFERON-γ USING PEG/K_3PO_4 AQUEOUS TWO-PHASE SYSTEM

EXTRACTION OF INTERFERON-γ USING PEG/K_3PO_4 AQUEOUS TWO-PHASE SYSTEM

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