关键词: Catharanthus roseus;shear stress;plant cell;bubble column

Abstract: In this paper,effects of increasing the height of column,bubble size and gas flow rate onthe growth of suspended Catharanthus roseus plant cells in a 1.5 litre bubble column were studied.Statistical characteristics of turbulence such as auto-correlation function,and auto-power spectral den-sity function of the nuctuating liquid velocity were measured in a bubble column employing pseudo-cell-granulated agar.Calculations of turbulent shear stress were carried out.From these studies it is sug-gested that in plant cell suspension culture sparged with large bubbles.turbulent shear stress is prob-ably one of the most important reasons for the inhibited cell growth and cell damage.Larger bubblesor higher gas flow rates lead to the increase in shear stress and are more detrimental to plant cells.

Key words: Catharanthus roseus, shear stress, plant cell, bubble column