储炬; 江洁; 李友荣; 俞俊棠
Chu Ju; Gang Jie; Li Yourong; Yu Juntang
摘要: Membrane microfiltration fermentation (MMF) with cell recycling was successfully applied to
the production of glucose oxidase (GOD). A plate microfiltration module was found suitable
for such purpose. By feeding whole medium in MMF, the productivity of GOD was much higher
than that by feeding glucose alone. With increasing dilution rate the enzyme productivity
increased and average enzyme activity decreased. The enzyme productivity of MMF under D =
0.12 h-1 and 0.20 h-1 were 3871 and 3945 U.h-1 respectively, which was about 3 times as
that of batch fermentation (BF) and the average enzyme activity was still as high as 37
U.mL-1 under D = 0.12 h-1. The relative efficiency of MMF applied to low yield strain was
higher than that applied to high yield strain.