CIESC Journal

• 化工学报 • 上一篇    下一篇

产壳聚糖酶菌种的选育和产酶条件的优化

朱江峰,郑连英   

  1. 浙江大学化学工程与生物工程系!杭州310027,浙江大学化学工程与生物工程系!杭州310027
  • 出版日期:2000-12-30 发布日期:2000-12-30

ISOLATION OF A CHITOSANASE-PRODUCING PENICILLIUM STRAIN AND OPTIMIZATION OF ITS FERMENTATION CONDITIONS

Zhu Jiangfeng and Zheng Lianying (Department of Chemical and Biochemical Engineering, Zhejiang University Hangzhou 310027)   

  • Online:2000-12-30 Published:2000-12-30

摘要: 从自然界中采集土壤样品,经筛选获得了一株产高活性壳聚糖酶的菌种,经初步鉴定属于青霉属。对该菌种进行紫外光诱变提高了它的产酶能力。通过Plackett-Burman设计初步优化了产酶条件,并研究了培养温度、摇床转速、培养基组成和装液量等培养条件对菌种产酶能力的影响,确定了该菌种的最佳产酶条件。在最佳培养条件下,壳聚精酶活最高可达216.6U/ml.

Abstract: A chitosanase-producing bacterium strain is isolated from soil. It was identified as Penicillium sp. Its chitosanase yield was improved by UV irradiation. The chitosanase fermentation condition for the mutant strain was first optimized with Plackett-Burman design and then the influence of the culture temperature, the rotating speed of the rime, the component of the culture medium and the medium volume were studied to optimize the fermentation conditions. A maximum yield of 216.6 U/ml was obtained.

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