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沈树宝; 柴本忠; 胡永红; 欧阳平凯
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SHEN Shubao;CHAI Benzhong;HU Yonghong;OUYANG Pingkai
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Abstract: In this work,the effect of alcohols (including alcohols containing single, two, three hydroxyl groups, respectively) on the enzyme- catalyzed synthesis of Z-L-Asp-PheOMe (ZAPM) is investigated by using a new technique of enzymatic catalysis in a heterogeneous pseudo-eutectic solid-to-solid mixture with thermolysin.The experimental result shows that the alcohols containing two hydroxyl groups are the most suitable adjuncts for the studied enzymatic synthesis system, and the yield of ZAPM for 1,4-butanediol as an adjunct reaches 95% at reaction time 2h, which is better than the results in two-phase systems and other pseudo-eutectic solid-to-solid mixture reported in literature.
沈树宝; 柴本忠; 胡永红; 欧阳平凯. 醇类助溶剂对拟低共熔体系中酶促合成ZAPM反应的影响 [J]. CIESC Journal.
SHEN Shubao;CHAI Benzhong;HU Yonghong;OUYANG Pingkai. EFFECT OF ALCOHOLS ON ENZYME-CATALYZED SYNTHESIS OF ZAPM IN PSEUDO-EUTECTIC MIXTURES OF SUBSTRATES [J]. .
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https://hgxb.cip.com.cn/CN/Y2001/V52/I12/1109