Abstract: A correlation for calculating the mass transfer coefficient of the adsorption process in the electrophoretic affinity chromatography (EAC) was established. Adsorption of human serum albumin (HSA) on Blue Sepharose Fast Flow(Blue)in alternating electric field was conducted at different electric field strength and alternating frequency to illustrate the effects of electroosmosis on the adsorption process of the EAC. The experimental results showed that the increase in current density led to corresponding increases in both electrosmotic flux and dynamic binding capacity. The mass transfer coefficient was obtained as a function of electric field strength, as indicated by current density, and alternating frequency. The occurrence of electrosmotic flux inside the porous gel particle greatly increased the speed of the migration of biological molecules towards the ligand immobilized in the inner surface of the particle and consequently resulted in an improved adsorption behavior, as shown by the increase in mass transfer coefficient. The results described in the present paper demonstrated the high potential of electroosmosis in the development of large scale electrophoretic chromatography of biological molecules.