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胰蛋白酶水解酪蛋白制备活性多肽的指数形式动力学新模型

何志敏; 齐崴; 何明霞   

  1. Chemical Engineering Research Center, Tianjin University, Tianjin 300072, China
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-10-28 发布日期:2002-10-28
  • 通讯作者: 何志敏

A Novel Exponential Kinetic Model for Casein Tryptic Hydrolysis to Prepare Active Peptides

HE Zhimin; QI Wei; HE Mingxia   

  1. Chemical Engineering Research Center, Tianjin University, Tianjin 300072, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-10-28 Published:2002-10-28
  • Contact: HE Zhimin

摘要: The kinetics of casein tryptic hydrolysis to prepare active peptides was investigated.
Taking intoaccount the reaction mechanism including single substrate hydrolysis,
irreversible enzyme inactivation, and substrateinhibition, a set of exponential equations
was established to characterize the enzymatic hydrolysis curves. Theverification was
carried out by a series of experimental results and indicated that the average regressive
error wasless than 5%. According to the proposed kinetic model, the kinetic constants and
thermodynamic constants of thereaction system were also calculated.

关键词: active peptide;casein;hydrolysis;kinetics;trypsin

Abstract: The kinetics of casein tryptic hydrolysis to prepare active peptides was investigated.
Taking intoaccount the reaction mechanism including single substrate hydrolysis,
irreversible enzyme inactivation, and substrateinhibition, a set of exponential equations
was established to characterize the enzymatic hydrolysis curves. Theverification was
carried out by a series of experimental results and indicated that the average regressive
error wasless than 5%. According to the proposed kinetic model, the kinetic constants and
thermodynamic constants of thereaction system were also calculated.

Key words: active peptide, casein, hydrolysis, kinetics, trypsin