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刘璘; 叶峰; 欧志敏
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LIU Lin;YE Feng;OU Zhimin
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Abstract: The enzymatic limited hydrolysis of soy protein isolate (SPI) by Alcalase 0.6L was carried out with pH-stat method at pH 8.0,50℃.The effects of the main parameters on the degree of hydrolysis, x , were investigated and a model based on the experiment results was deduced.The results showed that the substrate and the enzyme-substrate intermediate induced enzyme inhibition,which occurred due to free enzyme attacking the enzyme-substrate complexes.A critical 0 would correspond to a constant 0,which was 96.77mg·ml -1 under the experiment condition.The kinetic parameters calculated from the experiment data were K m=0.016mol·L -1 and V max =0.765mol·min·L -1 .The results showed that x can be regulated to about 6.0 with the mentioned factors in 30min.The good fitness between predicted and measured results confirmed the usefulness of the model.
刘璘; 叶峰; 欧志敏. 蛋白质酶法有限水解过程反应机理和动力学特性 [J]. CIESC Journal.
LIU Lin;YE Feng;OU Zhimin. MECHANISM AND KINETIC ANALYSIS OF ENZYMATIC LIMITED HYDROLYSIS OF SOY PROTEIN ISOLATE [J]. .
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https://hgxb.cip.com.cn/CN/Y2002/V53/I2/199