微囊化增殖酵母培养过程及动力学

CIESC Journal

微囊化增殖酵母培养过程及动力学

梅乐和; 姚善泾; 朱自强

浙江大学化学工程与生物工程学系

出版日期:2002-05-25 发布日期:2002-05-25

CULTIVATION AND KINETICS OF ENCAPSULATED Saccharomyces cerevisiate

MEI Lehe;YAO Shanjing;ZHU Ziqiang

Online:2002-05-25 Published:2002-05-25

摘要/Abstract

摘要： 研究了用NaCS -PDADMAC固定化酿酒酵母的培养过程 ,结果显示微囊化酿酒酵母的生长及产酒精情况与游离培养过程基本规律一致 ,微胶囊内酿酒酵母的最高细胞密度可达 2 6 4× 10 ¹⁰ ml^-1,表明NaCS -PDADMAC微胶囊适用于生物物质的固定化 ,酒精浓度可达到 4 7.0g·L^-1,微囊化酿酒酵母经连续发酵 16批后仍具有良好的性能 ,比普通连续培养高 5～ 6倍。在实验的基础上将酿酒酵母的游离培养动力学模型与微胶囊的物质传递相结合 ,建立了一个用于描述酿酒酵母微囊化培养生产酒精的模型 ,计算结果基本上能预测细胞密度、葡萄糖浓度和酒精浓度的变化趋势 .

Abstract: The cultivation of encapsulated S cerevisiate cells in NaCS-PDADMAC polyelectrolyte complexes was studied. The results showed that the encapsulated microorganisms had the same growth trends as in its free cell culture and thus NaCS-PDADMAC microcapsules were suitable for the encapsulation of biological substances. The encapsulated S cerevisiate cells were fermented sequentially in 16 batches. The highest cell density in the capsules reached 2 64×10 ¹⁰ cells·ml ^-1 and the ethanol concentration was 47 0 g·L ^-1 .A model of the cultivation of the encapsulated S cerevisiate was developed.

梅乐和; 姚善泾; 朱自强. 微囊化增殖酵母培养过程及动力学 [J]. CIESC Journal.

MEI Lehe;YAO Shanjing;ZHU Ziqiang. CULTIVATION AND KINETICS OF ENCAPSULATED Saccharomyces cerevisiate [J]. .