关键词: ginkgolide A;ginkgolide B;isolation and purification;uniform design;preparative liquid chromatog raphy

Abstract: In this paper, a simple preparative method for isolation and purification of ginkgolides A
and B was developed. As starting material, a commercially available standardized ginkgo
extract (EGb761, containing 24% flavonoid and 6% terpene trilactones) was used. After a
pretreatment step, optimized by the uniform design method, the concentrated intermediate
extract with high content of GA and GB (+90%) was separated into the individual terpenes by
preparative liquid chromatography eluted with petroleum ether-ethylacetate. Analysis of
products was carried out by means of HPLC-ELSD (evaporative light-scattering detector). The
results show that ginkgolides A and B are obtained in higher yield and better purity.

Key words: ginkgolide A, ginkgolide B, isolation and purification, uniform design, preparative liquid chromatog raphy