Ralstonia eutropha批式流加培养生产羟基丁酸-丙酸混合聚酯中丙酸进料优化研究

CIESC Journal

• RESEARCH NOTES • 上一篇下一篇

Ralstonia eutropha批式流加培养生产羟基丁酸-丙酸混合聚酯中丙酸进料优化研究

Longan SHANG^a,b; Jin Hwan DO^a; 范代娣^b; 姜岷^a; Ho Nam CHANG^a

^a Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science
^{and Technology,373-1 Kusong-dong, Yusong-gu, Taejeon 305-701, Korea
b} Department of Chemical Engineering, Northwest University, Xi’an 710069, China

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-04-28 发布日期:2003-04-28
通讯作者: Longan SHANG

ptimization of Propionic Acid Feeding for Production of Poly (3- hydroxybutyrat e- co- 3-
hydroxyvalerat e) in Fed-Batch Culture of Ralstonia eutropha

Longan SHANG^a,b; Jin Hwan DO^a; FAN Daidi^b; JIANG Min^a; Ho Nam CHANG^a

^a Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science
and Technology,373-1 Kusong-dong, Yusong-gu, Taejeon 305-701, Korea
^b Department of Chemical Engineering, Northwest University, Xi’an 710069, China

Received:1900-01-01 Revised:1900-01-01 Online:2003-04-28 Published:2003-04-28
Contact: Longan SHANG

摘要/Abstract

摘要： The feeding method of propionic acid for production of poly(3-hydroxybutyrate-co-3-hydro
xyvalerate) [P(3HB-co-3HV)] by fed-batch culture of Ralstonia eutropha was optimized to
achieve high cell density and high 3HV yield. Effects of different feeding strategies of
propionic acid on the production of P(3HB-co-3HV) were investigated. A decline of specific
synthesis rate of copolymer and the yield of 3HV unit from propionic acid were observed due
to the propionic acid accumulation in culture broth when the feeding solution with high
P/G(propionic acid to glucose) ratio was employed. It was further confirmed by controlling
propionic acid concentration at a low level in the separate feeding of propionic acid. An
optimal feeding strategy was demonstrated to reduce the propionic acid accumulation. The
cell concentration, P(3HB-co-3HV) productivity and 3HV unit fraction reached to 163.9 kg.m
-3,1.8 kg.m-3.h-1, and 10.6%(by mass), respectively, resulting in a yield of 0.33 g HV per
g propionic acid.

关键词: biodegradable polyester;feeding strategy;P(3HB-co-3HV);propionic acid;Ralstonia eutropha

Abstract: The feeding method of propionic acid for production of poly(3-hydroxybutyrate-co-3-hydro
xyvalerate) [P(3HB-co-3HV)] by fed-batch culture of Ralstonia eutropha was optimized to
achieve high cell density and high 3HV yield. Effects of different feeding strategies of
propionic acid on the production of P(3HB-co-3HV) were investigated. A decline of specific
synthesis rate of copolymer and the yield of 3HV unit from propionic acid were observed due
to the propionic acid accumulation in culture broth when the feeding solution with high
P/G(propionic acid to glucose) ratio was employed. It was further confirmed by controlling
propionic acid concentration at a low level in the separate feeding of propionic acid. An
optimal feeding strategy was demonstrated to reduce the propionic acid accumulation. The
cell concentration, P(3HB-co-3HV) productivity and 3HV unit fraction reached to 163.9 kg.m
-3,1.8 kg.m-3.h-1, and 10.6%(by mass), respectively, resulting in a yield of 0.33 g HV per
g propionic acid.

Key words: biodegradable polyester, feeding strategy, P(3HB-co-3HV), propionic acid, Ralstonia eutropha

Longan SHANG, Jin Hwan DO, 范代娣, 姜岷, Ho Nam CHANG. Ralstonia eutropha批式流加培养生产羟基丁酸-丙酸混合聚酯中丙酸进料优化研究[J]. CIESC Journal.

Longan SHANG, Jin Hwan DO, FAN Daidi, JIANG Min, Ho Nam CHANG. ptimization of Propionic Acid Feeding for Production of Poly (3- hydroxybutyrat e- co- 3-
hydroxyvalerat e) in Fed-Batch Culture of Ralstonia eutropha[J]. .

参考文献

[1] Anderson, A.J., Dawes, E.A., "Occurrence, metabolism, metabolic role, and industrial
uses of bacterial polyhydroxyalkanoates", Microbiol. Rev., 54, 450-472 (1990).
[2] Page, W.J., "Bacterial polyhydroxygalkanoates", Can. J.Microbiol., 41 (Suppl.1), 1-3
(1995).
[3] Lee, S.Y., "High cell-density culture of Escherichia coli ",Trends Biotechnol., 14, 98
-105 (1996).
[4] Kim, B.S., Lee, S.C., Lee, S.Y., Chang, H.N., Chang,Y.K., Woo, S.I., "Production of
poly(3-hydroxybutyric-co-3-hydroxywleric acid) by fed-batch culture of Alcaligenes
eutrophus with substrate control using on-line glucose analyzer", Enzyme Microb. Technol.,
16, 556-561 (1994).
[5] Du, G.C., Chen, J., Yu, J., Lun, S., "Feeding strategy of propionic acid for production
of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with Ralstonia eutropha", Biochem.Eng. J.,
8, 103-110 (2001).
[6] Choi, J.I., Lee, S.Y., "High-level production of poly(3-hydroxybutyrate-co-3-
hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli ", Appl. Environ.
Microbiol., 65, 4363-4368 (1999).
[7] Yim, K.S., Lee, S.Y., Chang, H.N., "Synthesis of poly-(3-hydroxybutyrate-co-3-
hydroxyvalerate) by recombinant Escherichia coli ", Biotechnol. Bioeng., 49, 495-503
(1996).
[8] Kobayashi, G., Tanaka, K., Itoh, H., Tsuge, T., Sonomoto, K., Ishizaki, A.,
"Fermentative production of P(3HB-co-3HV) from propionic acid by Alcaligenes eutrophus in
fedbatch culture with pH-stat continuous substrate feeding method", Biotechnol. Lett., 22,
1067-1069 (2000).
[9] Wang, J.P., Yu, J., "Kinetic analysis on inhibited growth and poly(3-hydroxybutyrate)
formation of alcaligenes eutrophus on acetate under nutrient-rich conditions",
Proc.Biochem., 36, 201-207 (2000).
[10] Braunegg, G., Sonnleitner, B., Lafferty. R.M., "A rapid gas chromatographic method for
the determination of Poly-β-hydroxybutyric acid in microbial biomass", European J. Appl.
Microbiol. Biotechnol., 6, 29-37 (1978).
[11] Ryu, H.W., Cho, K.S., Kim, B.S., Chang, Y.K.,Chang, H.N., Shim, H.J., "Mass production
of poly(3-hydroxybutyrate) by fed-batch cultures of Ralstonia eutropha with nitrogen and
phosphate limitation", J. Microbiol. Biotechnol., 9, 751-756 (1999).
[12] Doi, Y., Microbial polyesters, VCH, New York (1990).

Ralstonia eutropha批式流加培养生产羟基丁酸-丙酸混合聚酯中丙酸进料优化研究

ptimization of Propionic Acid Feeding for Production of Poly (3- hydroxybutyrat e- co- 3-
hydroxyvalerat e) in Fed-Batch Culture of Ralstonia eutropha

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价

Ralstonia eutropha批式流加培养生产羟基丁酸-丙酸混合聚酯中丙酸进料优化研究

ptimization of Propionic Acid Feeding for Production of Poly (3- hydroxybutyrat e- co- 3- hydroxyvalerat e) in Fed-Batch Culture of Ralstonia eutropha

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价

ptimization of Propionic Acid Feeding for Production of Poly (3- hydroxybutyrat e- co- 3-
hydroxyvalerat e) in Fed-Batch Culture of Ralstonia eutropha