CIESC Journal

• RESEARCH NOTES • 上一篇    下一篇

烟草过氧化物酶(TOPI)的分离与纯化的研究

夏炳乐; 刘清亮; 李敏莉; 徐小龙; 施春华; 解永树   

  1. Department of Chemistry, University of Science and Technology of China, Hefei 230026, China
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-06-28 发布日期:2003-06-28
  • 通讯作者: 夏炳乐

Separation and Purification of Tobacco Peroxidase I from Nicotiana Tobaccum

XIA Bingle; LIU Qingliang; LI Minli; XU Xiaolong; SHI Chunhua; XIE Yongshu   

  1. Department of Chemistry, University of Science and Technology of China, Hefei 230026, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-06-28 Published:2003-06-28
  • Contact: XIA Bingle

摘要: A new isoenzyme of tobacco peroxidase(TOP) I was purified from tobacco (K326) by using
acetonepowder, ammonium sulfate precipitation and column chromatography on DEAE-52
cellulose Sephadex G-75 andDEAE-Sephadex A-50. It is an iron-protein containing
haemachrome, whose molecular weight is 21888.5 and theisoelectric point is 3.5. The optimum
pH value and temperature of this enzyme is 6.0 and 45℃ respectively. Theenzyme is stable
in the pH range from 3.0 to 10.0 and has a favorable thermostability.

关键词: tobacco peroxidase;separation;purification

Abstract: A new isoenzyme of tobacco peroxidase(TOP) I was purified from tobacco (K326) by using
acetonepowder, ammonium sulfate precipitation and column chromatography on DEAE-52
cellulose Sephadex G-75 andDEAE-Sephadex A-50. It is an iron-protein containing
haemachrome, whose molecular weight is 21888.5 and theisoelectric point is 3.5. The optimum
pH value and temperature of this enzyme is 6.0 and 45℃ respectively. Theenzyme is stable
in the pH range from 3.0 to 10.0 and has a favorable thermostability.

Key words: tobacco peroxidase, separation, purification