宋宝东a; 丁辉a; 吴金川a; Hayashi Y.b; Talukder MMRb; 王世昌a
SONG Baodonga; DING Huia; WU Jinchuana; Hayashi Y.b; Talukder MMRb; WANG Shichanga
摘要: The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive
oil in two-phase system consisting of olive oil and phosphate buffer without organic
solvent. For both the coated and native lipases,the optimal buffer/oil volume ratio of 1.0,
aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the
coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated
lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the
final residual activity was 27% and 20% of their initial values, respectively. The final
substrate conversion by the coated lipase was ca 20% higher than that of the native lipase.