带环氧基的超顺磁性高分子微球的制备及其性能表征

CIESC Journal

• RESEARCH PAPERS • 上一篇下一篇

带环氧基的超顺磁性高分子微球的制备及其性能表征

刘先桥; 官月平; 邢建民; 马志亚; 刘会洲

Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical
Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing,100080,
China

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-12-28 发布日期:2003-12-28
通讯作者: 刘先桥

Synthesis and Properties of Micron-size Magnetic Polymer Spheres with Epoxy Groups

LIU Xianqiao; GUAN Yueping; XING Jianmin; MA Zhiya; LIU Huizhou

Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical
Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing,100080,
China

Received:1900-01-01 Revised:1900-01-01 Online:2003-12-28 Published:2003-12-28
Contact: LIU Xianqiao

摘要/Abstract

摘要： Micron-size superparamagnetic poly(styrene-divinylbenzene-glycidyl methacrylate) (PSt-DVB-
GMA)spheres were prepared via a modified suspension copolymerization method. Oleic acid
coated magnetite (Fe3O4)nanoparticles made by co-precipitation were first mixed with
monomers of St, DVB, GMA, and benzoyl peroxide (BPO) to form oil in water suspension with
the presence of poly(vinyl pyrrolidone) (PVP-K30) as a stabilizer.Then the temperature of
mixture was increased at a controlled rate to obtain small and relatively uniform
droplets.Finally, the copolymerization reaction was initiated by the decomposition of BPO.
The morphology and properties of magnetic PSt-DVB-GMA microspheres were examined by SEM,
TEM, VSM, XRD and FT-IR. The magnetic microspheres obtained have very small size (about 4-7
μm) in diameter with narrow size distribution and superparamagnetic characteristics.
Powder X-ray diffraction measurements show the inverse cubic spinel structure for the
magnetite dispersed in polymer microspheres. FT-IR spectroscopy indicates extensive oxirane
groups existed on the surface of magnetic PSt-DVB-GMA microspheres.

关键词: magnetic microsphere;synthesis;suspension polymerization;bioseparation

Abstract: Micron-size superparamagnetic poly(styrene-divinylbenzene-glycidyl methacrylate) (PSt-DVB-
GMA)spheres were prepared via a modified suspension copolymerization method. Oleic acid
coated magnetite (Fe3O4)nanoparticles made by co-precipitation were first mixed with
monomers of St, DVB, GMA, and benzoyl peroxide (BPO) to form oil in water suspension with
the presence of poly(vinyl pyrrolidone) (PVP-K30) as a stabilizer.Then the temperature of
mixture was increased at a controlled rate to obtain small and relatively uniform
droplets.Finally, the copolymerization reaction was initiated by the decomposition of BPO.
The morphology and properties of magnetic PSt-DVB-GMA microspheres were examined by SEM,
TEM, VSM, XRD and FT-IR. The magnetic microspheres obtained have very small size (about 4-7
μm) in diameter with narrow size distribution and superparamagnetic characteristics.
Powder X-ray diffraction measurements show the inverse cubic spinel structure for the
magnetite dispersed in polymer microspheres. FT-IR spectroscopy indicates extensive oxirane
groups existed on the surface of magnetic PSt-DVB-GMA microspheres.

Key words: magnetic microsphere, synthesis, suspension polymerization, bioseparation

刘先桥, 官月平, 邢建民, 马志亚, 刘会洲. 带环氧基的超顺磁性高分子微球的制备及其性能表征[J]. CIESC Journal.

LIU Xianqiao, GUAN Yueping, XING Jianmin, MA Zhiya, LIU Huizhou. Synthesis and Properties of Micron-size Magnetic Polymer Spheres with Epoxy Groups[J]. .

参考文献

[1] Safarik, I., Safariková, M., "Use of magnetic techniques for the isolation of cells",
J. Chromatogr. B, 722 (1-2), 33-53 (1999).
[2] Bilková, Z., Slovákova, M., Horák, D., Lenfeld, J.,Churácek, J., "Enzymes immobilized
on magnetic carriers:efficient and selective system for protein modification",
J.Chromatogr. B, 770 (1-2), 177-181 (2002).
[3] Kronick, P., Gilpin, R.W., "Use of superparamagnetic particles for isolation of cells",
J. Biochem. Biophys. Methods,12 (1), 73-80 (1936).
[4] Cupta, P.K., Hung, C.T., "Magnetically controlled targeted micro-carrier systems", Life
Sci., 44 (2), 175-186 (1989).
[5] Chatterjee, J., Haik, Y., Chen, C.J., "Modification and characterization of
polystyrene-based magnetic microspheres and comparison with albumin-based magnetic
microspheres", J. Magn. Magn. Mater., 225 (1), 21-29(2001).
[6] Denkbas, E.B., Kilicay, E., Birlikseven, C., Oztiirk, E.,"Magnetic chitosan
microspheres: preparation and characterization", React. Func. Polym., 50 (3), 225-232
(2002).
[7] Cocker, T.M., Fee, C.J., Evans, R.A., "Preparation of magnetically susceptible
polyacrylamide/magnetite beads for use in magnetically stabilized fluidized bed
chromatography", Biotechnol. Bioeng., 53 (1), 79-87 (1997).
[8] Horák, D., "Magnetic polyglycidylmethacrylate microspheres by dispersion
polymerization", J. Polym. Sci. A,39 (21), 3707-3715 (2001).
[9] Li, X.H., Sun, Z.H., "Synthesis of magnetic polymer microspheres and application for
immobilization of proteinase of balillus sublitis", J. Appl. Polym. Sci., 58 (11), 1991-
1997(1995).
[10] Ugelstad, J., Ellingsen, T., Berge, A., Helgee, B., "Magnetic polymer particles", WO
Pat. 8303920 (1983).
[11] Ding, X., Sun, Z., Wang, G., "Characterization of Fe3O4/poly(styrene-co-N-
isopropylacrylamide) magnetic particles with temperature sensitivity", Chin. J. Polym.Sci.,
17 (2), 129-134 (1999).
[12] Khng, H.P., Cunliffe, D., Davies, S., Turner, N.A., Vulfson,E.N., "The synthesis of
sub-micron magnetic particles and their use for preparative purification of proteins",
Biotech.Bioeng., 60 (4), 419-424 (1998).
[13] Yanase, N., Noguchi, H., Asakura, H., Suzuta, T., "Preparation of magnetic latex
particles by emulsion polymerization of styrene in the presence of a ferrofiuid", J.
Appl.Polym. Sci., 50 (5), 765-776 (1993).
[14] Kondo, A., Kamura, H., Higashitahi, K., "Development and application of thermosenstive
magnetic immunomicrospheres for antibody purification", Appl. Microbiol.Biotechnol., 41
(1), 99-105 (1994).
[15] Shen, L., Laibinis, P.E., Hatton, T.A., "Aqueous magnetic fluids stabilized by
surfactant bilayers", J. Magn. Magn.Mater., 194 (1-3), 37-44 (1999).
[16] Guan, Y.P., "Synthesis, characterization and applications of superparamagnetic polymer
nanostructured carriers for affinity separations", Ph.D. Thesis, Institute of Process
Engineering, CAS, (China), 26-39 (2000). (in Chinese)
[17] Ma, G.H., Polymer Interfaces and Emulsions, Marcel Dekker Inc., New York, 80-82
(1999).
[18] Horák, D., Shapoval, P., "Reactive poly(glycidyl methacrylate) microspheres prepared
by dispersion polymerization",J. Polym. Sci. A, 38 (21), 3855-3863 (2000).
[19] Svec, F., Kalal, J., Menyailova, I.I., Nakhapetyan, L.A.,"Immobilization of
amyloglucosidase on poly[(glycidyl methacrylate)co(ethylene dimethacrylate)] carrier and
its derivatives", Biotechnol Bioeng., 20 (9), 1319-1328(1978).
[20] Shmanai, V.V., Bylina, G.S., "Protein immobilization on formylated polystyrene
supports", React. Func. Polym.,43 (3), 243-251 (2000).

带环氧基的超顺磁性高分子微球的制备及其性能表征

Synthesis and Properties of Micron-size Magnetic Polymer Spheres with Epoxy Groups

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价