关键词:

聚氨酯泡沫, hEGF重组菌, 固定化培养 , 质粒稳定性

Abstract: A genetic recombinant hEGF producer E.coli JM101 was immobilized in polyurethane foam matrix and a three-phase fluidized-bed bioreactor was used to produce human epidermal growth factor (hEGF).The results indicated that polyurethane foam is an adequate immobilization material. With the addition of polyurethane foam matrix, the free cell density, plasmid stability and hEGF productivity were higher than those without the foam matrix, respectively. The two-stage culture of the immobilized cells was further carried out, in which, the first stage was performed in a three-phase fluidized-bed bioreactor for cell growth and the second stage was performed in bubbling bed for product expression. At a dilution rate of 0.2h^-1, after continuous cultivation for more than 60h, the free cell density and product expression were maintained relatively stable. The hEGF productivity was still higher than 140mg•L^-1 and the fraction of plasmid-carrying was more than 27%. The hEGF productivity in two-stage process was higher than the batch process.

Key words:

聚氨酯泡沫, hEGF重组菌, 固定化培养 , 质粒稳定性