Abstract: A CFD simulation of liquid flow in porous media was developed. The effects of electric field strength and the porosity of media on electroosmotic flow and mass transport were presented. The CFD simulation results were compared to the experimental data obtained in electrochromatography on hydroxyapatite, ion exchange electrochromatography on DEAE Sepharose Fast Flow, and affinity electrochromatography on Blue Sepharose Fast Flow. The good agreement between the numerical simulation with the experimental results including the overall electroosmotic flux and the mass transport described by mass transport coefficient and breakthrough curve confirms the validity of the CFD simulation and indicates the high potential of electroosmosis in the development of new solid-liquid separation processes.