关键词:

谷氨酸棒杆菌NS 611, 谷氨酰胺, 原位氮饥饿, 梯度补氮, 谷氨酰胺合成酶

Abstract: The effects of uniform and gradient fed nitrogen on glutamine synthetase(GS),glutamate dehydrogenase(GDH)and glutamate synthase(GOGAT)were investigated in glutamine production by fermentation of Corynebacterium glutamicum NS611 after 3 h of in-situ nitrogen starvation. It was shown that the strain in the later growth phase entered naturally into in-situ nitrogen starvation by controlling the initial concentration of urea and the biomass was slightly decreased. The pH value reached 6.5 again in the culture system, which confirmed the beginning of nitrogen starvation in the culture system. After 3 h nitrogen starvation the activity of GS was increased over two folds and the time of high activity of GS persisted three folds longer in the gradient fed nitrogen system than that in the normal fed batch. The higher activity of GDH was also maintained. The glutamine production increased by 72% than the original technology of nitrogen starvation and the time of fermentation was shortened by above 12 h.

Key words:

谷氨酸棒杆菌NS 611, 谷氨酰胺, 原位氮饥饿, 梯度补氮, 谷氨酰胺合成酶