Abstract: It is difficult to study the metabolic behavior of filamentous microorganisms in traditional solid-state fermentation systems,due to the tight integration between mycelia and medium.It is not possible either to study the kinetics of feeding and continuous strategy in this system, since the area of medium surface would change with the inflow of fresh medium.Aiming at avoiding these difficulties,a novel membrane culture system was developed.The membrane and microorganisms were transferred from one plate to another to change the medium.Another modified membrane culture system was studied by replacing agar with absorbent cotton to simplify the operation in laboratory conditions.Coniothyrium minitans was used as a model filamentous fungus to simplify the research, since the improvement of conidial production was the major aim in solid-state culture of this fungus.Production of biomass and conidia，distribution of conidia in medium, and change of pH during cultivation period were analyzed to evaluate the applicability of this novel membrane culture system.Results showed the mycelial penetration of C.minitans into the medium by using a cellulose acetate membrane with pore size of 0.45 μm, whereas the biomass production and conidial production were not affected.The conidial production was improved by 1.8-fold when the medium contents were changed during the cultivation period.The modified membrane culture system provided similar results as PDA, whereas it is more feasible and simple for the research on continuous solid-state cultivation.This is the first report on the potential of membrane cultivation system in fed-batch or continuous cultivation of C.minitans in solid-state fermentation in laboratory conditions.The studied membrane culture system has great potential in the solid-state fermentation of other filamentous microorganisms.