Abstract: A new membrane electrolyte assembly (MEA) preparation method for polymer electrolyte membrane fuel cell (PEMFC) was developed by applying the directly printing catalyst on membrane technique.This method was simple and easy to be controlled as verified by repetition experiment. When the membrane with catalyst prepared by the new technique and the electrode with diffusion layer was only sandwiched but not hot pressed, this kind of MEA was called not-hot-press MEA (NPMEA) and its fuel cell performance was better than that of MEA which was hot pressed (HPMEA).The effects of 6 different kinds of solvents in catalyst mixture ink on the performance of fuel cell were assessed.It was discovered that iso-propanol was the best solvent in catalyst mixture ink and showed the best performance of fuel cell. Finally several MEAs prepared by different ways were tested on fuel cell station and it was reported that the performance of MEA prepared by the directly printing catalyst on membrane technique was the best in the whole voltage region.