阿魏酸对蘑菇酷氨酸酶的抑制效应

CIESC Journal

• REACTION KINETICS, CATALYSIS AND…… • 上一篇下一篇

阿魏酸对蘑菇酷氨酸酶的抑制效应

龚盛昭^a,b; 程江^a; 杨卓如^a

^a School of Chemical ＆ Energy Engineering, South China University of Technology, Guangzhou 510640, China
^b Department of Chemical Engineering,Guangdong Industry Technical College, Guangzhou 510300, China

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-12-28 发布日期:2005-12-28
通讯作者: 龚盛昭

Inhibitory Effect of Ferulic Acid on Oxidation of L-DOPA Catalyzed by Mushroom Tyrosinase

GONG Shengzhao^a,b; CHENG Jiang^a; YANG Zhuoru^a

^a School of Chemical ＆ Energy Engineering, South China University of Technology, Guangzhou 510640, China
^b Department of Chemical Engineering,Guangdong Industry Technical College, Guangzhou 510300, China

Received:1900-01-01 Revised:1900-01-01 Online:2005-12-28 Published:2005-12-28
Contact: GONG Shengzhao

摘要/Abstract

摘要： The inhibitory effect of ferulic acid on the diphenolase activity of mushroom tyrosinase and the kinetic behavior were studied with L-3,4-dihydroxyphenylalanine （L-DOPA） as substrate. The inhibitor concentration leading to 50% relative activity lost （IC50） was estimated to be 0.15 mmol·L^-1. The inhibition mechanism obtained from Lineweaver-Burk plots shows that ferulic acid is a competitive inhibitor and the inhibition of tyrosinase by ferulic acid is a reversible reaction. The equilibrium constant for ferulic acid binding with the tyrosinase was determined to be 0.25 mmol·L^-1 for diphenolaase.

关键词: 阿魏酸;蘑菇酪氨酸酶;抑制效应;L-DOPA催化;L-3;4-二羟(基);苯丙氨酸

Abstract: The inhibitory effect of ferulic acid on the diphenolase activity of mushroom tyrosinase and the kinetic behavior were studied with L-3,4-dihydroxyphenylalanine （L-DOPA） as substrate. The inhibitor concentration leading to 50% relative activity lost （IC50） was estimated to be 0.15 mmol·L^-1. The inhibition mechanism obtained from Lineweaver-Burk plots shows that ferulic acid is a competitive inhibitor and the inhibition of tyrosinase by ferulic acid is a reversible reaction. The equilibrium constant for ferulic acid binding with the tyrosinase was determined to be 0.25 mmol·L^-1 for diphenolaase.

Key words: tyrosinase, ferulic acid, kinetics, inhibition, L-DOPA, diphenolase

龚盛昭,程江,杨卓如. 阿魏酸对蘑菇酷氨酸酶的抑制效应[J]. CIESC Journal.

GONG Shengzhao, CHENG Jiang, YANG Zhuoru. Inhibitory Effect of Ferulic Acid on Oxidation of L-DOPA Catalyzed by Mushroom Tyrosinase[J]. .

阿魏酸对蘑菇酷氨酸酶的抑制效应

Inhibitory Effect of Ferulic Acid on Oxidation of L-DOPA Catalyzed by Mushroom Tyrosinase

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