L-瓜氨酸酶法制备中精氨酸脱亚氨酸酶的活性与稳定性研究

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L-瓜氨酸酶法制备中精氨酸脱亚氨酸酶的活性与稳定性研究

李加友; 曹瑜; 刘毅; 钱绍松; 焦庆才

State Key Laboratory for Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, China

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-12-28 发布日期:2005-12-28
通讯作者: 李加友

Activity and Stability of Arginine Deiminase for Producing L-citrulline

LI Jiayou; CAO Yu; LIU Yi; QIAN Shaosong; JIAO Qingcai

State Key Laboratory for Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, China

Received:1900-01-01 Revised:1900-01-01 Online:2005-12-28 Published:2005-12-28
Contact: LI Jiayou

摘要/Abstract

摘要： A novel Enterococcus faecalis strain designated N J402 was found with high activity of arginine deiminase （ADI）. The optimum condition for catalytic activity was determined in terms of temperature （about 40℃）, thermostability （available 37℃） and pH （6-7）. The effects of substrate and product concentration were studied. The effects of various metal ions added in reaction mixtures on the biocatalyst were investigated and ADI of N J402 was found to exhibit Co^2＋ dependence, different from previous reports. Surfactant, cetyl trimethyl ammonium bromide, was one of the most important keys for producing L-citrulline. The enzyme in resting cells possessed the quality of high stability for reuse.

关键词: L-瓜氨酸酶;制备方法;精氨酸脱亚氨酸酶;活性;稳定性

Abstract: A novel Enterococcus faecalis strain designated N J402 was found with high activity of arginine deiminase （ADI）. The optimum condition for catalytic activity was determined in terms of temperature （about 40℃）, thermostability （available 37℃） and pH （6-7）. The effects of substrate and product concentration were studied. The effects of various metal ions added in reaction mixtures on the biocatalyst were investigated and ADI of N J402 was found to exhibit Co^2＋ dependence, different from previous reports. Surfactant, cetyl trimethyl ammonium bromide, was one of the most important keys for producing L-citrulline. The enzyme in resting cells possessed the quality of high stability for reuse.

Key words: L-citrulline, L-arginine, arginine deiminase, activity, optimization

李加友,曹瑜,刘毅,钱绍松,焦庆才. L-瓜氨酸酶法制备中精氨酸脱亚氨酸酶的活性与稳定性研究[J]. CIESC Journal.

LI Jiayou, CAO Yu, LIU Yi, QIAN Shaosong, JIAO Qingcai. Activity and Stability of Arginine Deiminase for Producing L-citrulline[J]. .

L-瓜氨酸酶法制备中精氨酸脱亚氨酸酶的活性与稳定性研究

Activity and Stability of Arginine Deiminase for Producing L-citrulline

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