Abstract: Metabolic flux analysis (MFA) was used to study the relationships among different metabolic pathways in the fermentation of Corynebacterium glutamicum CCTCC M201005.Metabolic network models were established to study the fermentation behavior under different fermentation stages and different dissolved oxygen tension (DOT) levels in batch fermentation.The effects of metabolic node on REA-11 secretion were evaluated.In addition, the supply and consumption of energy and reducing equivalent in cell metabolism were analyzed as well.The results showed that the flux of Phosphate-pentose Pathway (PP) was maintained at a high level during the whole fermentation process, indicating PP played a key role in the metabolism of C. glutamicum CCTCC M201005. The flux of REA-11 decreased when DOT increased, whereas cell synthesis flux showed a reverse trend.Increase of ATP flux stimulated cell growth but decreased REA-11 production.