Abstract: A new extraction process, named high pressure extraction (HPE) at room temperature, was used to extract ginsenoside from ginseng. The extraction process of HPE was optimized with the uniform test design method. The optimum HPE process conditions of ginsenoside were: solvent 50% ethanol, pressure 200 MPa, ratio of raw ginseng(g) to solvent(ml) 1∶100, extraction time 1 min,yield 7.32%. Compared with the cooking,reflux extraction,ultrasonic and supercritical CO2 extraction processes, HPE have many advantages, such as higher yield, no heating, shorter extracting time and energy saving. HPE provides a new process for the extraction of ginsenoside from ginseng at room temperature.