Abstract: The aim of the study was to optimize polymerization conditions so as to maximize the binding capacity for proteins, using BSA as a model substance.A macro-porous hydrophilic bead copolymer was synthesized by organic two-phase suspension polymerization,with glycidyl methacrylate and acrylamide as monomers, N,N′-methylene-bis(acrylamide) as a crosslinker and formamide as the dispersion phase, behaving as porogenic agent, the continuous phase being n-heptane. The characteristic features of the copolymer were studied, including specific surface area, pore diameter, pore volume and distribution, and the content of oxirane groups as well.It was known from past experience that the cross-linking degree, amount of GMA and amount of formamide were the three main factors affecting the BSA adsorption capacity.For exploring their effects and all possible interactions, a 2³ complete factorial design was used in experiment.Its analysis showed their effects in the order:cross-linkage>GMA amount>formamide amount, while the three two-factor interactions and the three-factor interaction were attributable to experimental error or noise.Consequently the estimated response(BSA adsorption capacity) could be expressed with a linear function of three main variables.Follow-up investigations found maximum adsorption capacity of 25.6 mg·g^-1 wet resin against the original 9.1 mg·g^-1 wet resin.