关键词: 柔性固定化, 双醛淀粉, 木瓜蛋白酶, Mannich反应, 胺化聚苯乙烯

Abstract: In this paper the flexible immobilization model of enzyme is presented.A new amino polystyrene (0.4—6.0 mmol NH₂•g^-1) was prepared by Mannich reaction as immobilization support, DAS was used as the flexible chain,and then papain was immobilized. The recovery of immobilized enzyme activity could reach 40%—50%,equal to 1.8—2.4 times that of arm spacer support, and the stability of immobilized enzyme was good.The flexible immobilized model could achieve a higher recovery of immobilized enzyme activity than traditional covalent immobilization and arm spacer immobilization.

Key words: 柔性固定化, 双醛淀粉, 木瓜蛋白酶, Mannich反应, 胺化聚苯乙烯