Production of 2,3-Butanediol by Klebsiella Pneumoniae Using Glucose and Ammonium Phosphate

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Production of 2,3-Butanediol by Klebsiella Pneumoniae Using Glucose and Ammonium Phosphate

QINJiayang(秦加阳),XIAOZijun(肖梓军),MACuiqing(马翠卿),XIENengzhong(谢能中),LIUPeihai(刘培海)andXUPing(许平)

State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-02-28 发布日期:2006-02-28
通讯作者: XU Ping(许平)

Production of 2,3-Butanediol by Klebsiella Pneumoniae Using Glucose and Ammonium Phosphate

Received:1900-01-01 Revised:1900-01-01 Online:2006-02-28 Published:2006-02-28

摘要/Abstract

摘要： The production of 2,3-butanediol by Klebsiella pneumoniae from glucose supplemented with different salts was studied. A suitable medium composition was defined by response surface experiments. In a medium containing glu-cose and (NH4)2HPO4, the strain could convert 137.0g of glucose into 52.4g of 2,3-butanediol and 8.4g of acetoin in shaking flasks. The diol yield amounted to 90% of its theoretical value and the productivity was 1—1.5g?L－1?h－1. In fed-batch fermentation, the yield and productivity of diol were further enhanced by maintaining the pH at 6.0. Up to 92.4g of 2,3-butanediol and 13.1g of acetoin per liter were obtained from 215.0g of glucose per liter. The diol yield reached 98% of its theoretical value and the productivity was up to 2.1g?L－1?h－1.

关键词: 2;3-butanediol production;optimization;fed-batch;Klebsiella pneumoniae

Key words: 2, 3-butanediol production, optimization, fed-batch, Klebsiella pneumoniae

QINJiayang(秦加阳),XIAOZijun(肖梓军),MACuiqing(马翠卿),XIENengzhong(谢能中),LIUPeihai(刘培海)andXUPing(许平). Production of 2,3-Butanediol by Klebsiella Pneumoniae Using Glucose and Ammonium Phosphate[J]. CIESC Journal.

参考文献

1    Syu, M.J., “Biological production of 2,3-butanediol”, Appl. Microbiol. Biotechnol., 55, 10—18 (2001).
2    Garg, S.K., Jain, A., “Fermentative production of 2,3-butanediol: A review”, Bioresource Technol., 51, 103—109 (1995).
3    Perego, P., Converti, A., Borghi, M.D., “Effects of tem-perature, inoculum size and starch hydrolyzate concen-tration on butanediol production by Bacillus licheni-formis”, Bioresource Technol., 89, 125—131 (2003).
4    Flickinger, M.C.,  “Current biological research in con-version of cellulosic carbohydrates into liquid fuels: How far have we come”, Biotechnol. Bioeng., 22 (suppl.), 27—48 (1980).
5    Magee, R.J., Kosaric, N., “The microbial production of 2,3-butanediol”, Adv. Appl. Microbiol., 32, 89—161 (1987).
6    Olson, B.H., Johnson, M.J., “The production of 2,3-butylene glycol by Aerobacter aerogenes”, J. bacte-rial., 55, 209—222 (1948).
7    Yu, E.K.C., Saddler, J.N., “Fed-batch approach to pro-duction of 2,3-butanediol by Klebsiella pneumoniae grown on high substrate concentration”, Appl. Environ. Microbial., 46, 630—635 (1983).
8    Zeng, A.P., Biebl, H., Deckwer, W.D., “Production of 2,3-butanediol in a membrane bioreactor with cell recy-cle”, Appl. Microbiol. Biotechnol., 34, 463—468 (1991).
9    Afschar, A.S., Bellgardt, K.H., Vaz, Rossell, C.E., Czok, A., Schaller, K., “The production of 2,3-butanediol by fermentation of high test molasses”, Appl. Microbiol. Biotechnol., 34, 582—585 (1991).
10    Nakashimada, Y., Marwoto, B., Kashiwamura, T., Ka-kizono, T., Nishio, N., “Enhanced 2,3-butanediol pro-duction by addition of acetic acid in Paenibacillus po-lymyxa”, J. Biosci. Bioeng., 90, 661—664 (2000).
11    Pirt, S.J., Callow, D.S., “Production of 2,3-butanediol by Aerobacter aerogenes in a single stage process”, J. Appl. Bacteriol., 21, 188—205 (1958).
12    Gu, J.S., Xu, P., Qu, Y.B., “A biocatalyst for pyruvate preparation from DL-lactate: Lactate oxidase in a Pseu-domonas sp.”, J. Mol. Catal. B: Enzym., 18, 299—305 (2002).
13    Long, S.K., Patrick, R., “The present status of the 2,3-butylene glycol fermentation”, Adv. Appl. Microbiol., 5, 135—155 (1963).
14    Ui, S., Matsuyama, N., Masuda, H., Muraki, H., “Mechanism for the formation of 2,3-butanediol stereoi-somers in Klebsiella pneumoniae”, J. Ferm. Technol., 62, 551—559 (1984).
15    Ronkainen, H., Koskinen, J., Varjus, S., Holmberg, K., “Experimental design and modeling in the investigation of process parameter effects on the tribological and me-chanical properties of r.f.-plasma-deposited and C:H films”, Surf. Coat. Tech., 122, 150—160 (1999).
16    Grover, B.S., Garg, S.K., Verma, J., “Prodution of 2,3-butanediol from wood hydrolysate by Klebsiella pneumoniae”, World J. Microbiol. Biotechnol., 6, 328—332 (1990).
17    Blomqvist, K., Nikkola, M., Lehtovaara, P., Suihko, M., Airaksinen, U., Straby, K., Knowles, J., Penttila, M., “Characterization of the genes of the 2,3-butanediol op-erons from Klebsiella terrigena and Enterobacter aero-genes”, J. Bacteriol., 175, 1392—1404 (1993).
18    Juni, E., “Mechanisms of formation of acetoin by bacte-ria”, J. Biol. Chem., 195, 715—726 (1952).
19    Poulsen, C., Stougaard, P., “Purification and properties of Saccharomyces cerevisiae acetolactate synthase from recombinant Escherichia coli”, Eur. J. Bioch., 185,     433—439 (1989).
20    Ramachandran, K.B., Goma, G., “Effects of oxygen supply and dilution rate on the production of 2,3-butanediol in continuous bioreactor by Klebsiella pneumoniae”, Enzym. Microb. Technol., 9, 107—111 (1987).
21    Ramachandran, K.B., Goma, G., “2,3-Butanediol pro-duction from glucose by Klebsiella pneumoniae in a cell recycle system”, J. Biotechnol., 9, 39—46 (1988).

Production of 2,3-Butanediol by Klebsiella Pneumoniae Using Glucose and Ammonium Phosphate

Production of 2,3-Butanediol by Klebsiella Pneumoniae Using Glucose and Ammonium Phosphate

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