渗透压调节剂对耐高渗菌Candida Krusei生产甘油的影响

CIESC Journal

• BIOTECHNOLOGY & BIOENGINEERING • 上一篇下一篇

渗透压调节剂对耐高渗菌Candida Krusei生产甘油的影响

陈国; 姚善泾; 关怡新

Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-06-28 发布日期:2006-06-28
通讯作者: 陈国

Influence of Osmoregulators on Osmotolerant Yeast Candida krusei for the Production of Glycerol

CHENGuo; YAOShanjing; GUANYixin

Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China

Received:1900-01-01 Revised:1900-01-01 Online:2006-06-28 Published:2006-06-28
Contact: CHEN Guo

摘要/Abstract

摘要： Candida krusei was osmotolerant yeast for the production of glycerol. Extracellular osmotic pressure was one of the key factors to induce the enzyme activity of glycerol-3-phosphate dehydrogenase(GPDH) which severely affected the glycerol productivity. Three osmoregulators such as NaCl, PEG4000 and glycerol were used to investigate their effects on yeast growth, glucose consumption and glycerol production. In order to determine the effect of extracellular glycerol concentration, different amounts of glycerol were initially supplemented as an osmoregulator to increase glycerol production. The maximum glycerol concentration attained 179gL－1 with initial glycerol concentration of 80gL－1 in medium, compared with 41gL－1 in the control experiment. These results were successfully reproduced for fed-batch process in an air-lift reactor.

关键词: Candida krusei;glycerol;osmotolerant yeast;osmoregulator;air-lift reactor

Abstract: Candida krusei was osmotolerant yeast for the production of glycerol. Extracellular osmotic pressure was one of the key factors to induce the enzyme activity of glycerol-3-phosphate dehydrogenase(GPDH) which severely affected the glycerol productivity. Three osmoregulators such as NaCl, PEG4000 and glycerol were used to investigate their effects on yeast growth, glucose consumption and glycerol production. In order to determine the effect of extracellular glycerol concentration, different amounts of glycerol were initially supplemented as an osmoregulator to increase glycerol production. The maximum glycerol concentration attained 179gL－1 with initial glycerol concentration of 80gL－1 in medium, compared with 41gL－1 in the control experiment. These results were successfully reproduced for fed-batch process in an air-lift reactor.

Key words: Candida krusei, glycerol, osmotolerant yeast, osmoregulator, air-lift reactor

陈国, 姚善泾, 关怡新. 渗透压调节剂对耐高渗菌Candida Krusei生产甘油的影响[J]. CIESC Journal.

CHENGuo, YAOShanjing, GUANYixin. Influence of Osmoregulators on Osmotolerant Yeast Candida krusei for the Production of Glycerol[J]. .

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渗透压调节剂对耐高渗菌Candida Krusei生产甘油的影响

Influence of Osmoregulators on Osmotolerant Yeast Candida krusei for the Production of Glycerol

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